背景:锦鸡儿属包括多种具有药用和生态价值的植物物种。然而,锦鸡儿的一些物种在形态上非常相似,因此,根据其形态特征来鉴定该属中的物种是相当复杂的。在我们的研究中,采用lumina配对末端测序技术研究了锦鸡儿和锦鸡儿的遗传组织和结构,包括先前发表的7种锦鸡儿植物的叶绿体基因组序列。
结果:西藏C.tica和土耳其C.rukestanica叶绿体基因组的长度分别为128,433bp和129,453bp,分别。这两个物种中不存在反向重复序列,将它们归类为反向重复丢失进化枝(IRLC)。它们编码110和111个基因(4/4rRNA基因,30/31tRNA基因,和76/76蛋白质编码基因),分别。将西藏C.tica和Turkestanica的叶绿体基因组与其他7种锦鸡儿属的叶绿体基因组进行比较,显示出很高的整体序列相似性。然而,在某些基因间区域之间观察到一些差异(matK-rbcL,psbD-psbM,atpA-psbI,等。).核苷酸多样性(π)分析揭示了五个高度可能的可变区的检测,即rps2-atpI,accD-psaI-ycf4,cemA-peta,psbN-psbH和rpoA-rps11。系统发育分析显示,西藏锦鸡儿的姊妹物种是胡巴塔锦鸡儿,而C.turkestanica的近亲是锦鸡儿。
结论:本研究提供了有价值的信息,介绍了西藏梭菌和土耳其梭菌的叶绿体基因组,这有助于锦鸡儿的鉴定和分类。
BACKGROUND: The genus
Caragana encompasses multiple plant species that possess medicinal and ecological value. However, some species of
Caragana are quite similar in morphology, so identifying species in this genus based on their morphological characteristics is considerably complex. In our research, illumina paired-end sequencing was employed to investigate the genetic organization and structure of Caragana tibetica and
Caragana turkestanica, including the previously published chloroplast genome sequence of 7
Caragana plants.
RESULTS: The lengths of C. tibetica and C. turkestanica chloroplast genomes were 128,433 bp and 129,453 bp, respectively. The absence of inverted repeat sequences in these two species categorizes them under the inverted repeat loss clade (IRLC). They encode 110 and 111 genes (4 /4 rRNA genes, 30 /31tRNA genes, and 76 /76 protein-coding genes), respectively. Comparison of the chloroplast genomes of C. tibetica and C. turkestanica with 7 other Caragana species revealed a high overall sequence similarity. However, some divergence was observed between certain intergenic regions (matK-rbcL, psbD-psbM, atpA-psbI, and etc.). Nucleotide diversity (π) analysis revealed the detection of five highly likely variable regions, namely rps2-atpI, accD-psaI-ycf4, cemA-petA, psbN-psbH and rpoA-rps11. Phylogenetic analysis revealed that C. tibetica\'s sister species is Caragana jubata, whereas C. turkestanica\'s closest relative is
Caragana arborescens.
CONCLUSIONS: The present study provides worthwhile information about the chloroplast genomes of C. tibetica and C. turkestanica, which aids in the identification and classification of Caragana species.