据报道,大麻素具有神经保护特性,在体外和体内模型中在神经发生和神经可塑性中起作用。大麻酚(CBN)是大麻中Δ9-四氢大麻酚降解产生的次要大麻素,并具有抗氧化剂,镇痛药,抗菌,和抗炎作用。在这项研究中,我们通过MTT分析和下一代转录组测序分析探讨了20µMCBN(6.20µg/mL)对分化的NSC-34细胞的生物学效应.已经进行了KEGG和基因本体论富集分析以评估潜在的CBN相关过程。我们的结果强调了CBN没有任何细胞毒性作用。比较转录组分析指出Cdkn2a的下调,Cdkn2c和Cdkn2d基因,已知抑制细胞周期。Ccne2,Cdk2,Cdk7,Anapc11,Anapc10,Cdc23,Cdc16,Anapc4,Cdc27,Stag1,Smc3,Smc1a,Nipbl,Pds5a,Pds5b,和Wapl基因,以其作为细胞周期进程激活剂的作用而闻名,反而被上调了。我们的工作表明,CBN调节与细胞周期相关的许多基因的表达,轴突成熟所需要的,迁移,和突触可塑性,而不影响参与细胞死亡或肿瘤发生的基因的表达。
Cannabinoids are reported to have neuroprotective properties and play a role in neurogenesis and neuroplasticity in in vitro and in vivo models.
Cannabinol (CBN) is a minor cannabinoid produced by the degradation of Δ9-tetrahydrocannabinol in Cannabis sativa L. and exhibits anti-oxidant, analgesic, anti-bacterial, and anti-inflammatory effects. In this study, we explored the biological effects of 20 µM CBN (6.20 µg/mL) on differentiated NSC-34 cells by MTT assay and next-generation sequencing analysis on the transcriptome. KEGG and Gene Ontology enrichment analyses have been performed to evaluate potential CBN-associated processes. Our results highlighted the absence of any cytotoxic effect of CBN. The comparative transcriptomic analysis pointed out the downregulation of Cdkn2a, Cdkn2c and Cdkn2d genes, which are known to suppress the cell cycle. Ccne2, Cdk2, Cdk7, Anapc11, Anapc10, Cdc23, Cdc16, Anapc4, Cdc27, Stag1, Smc3, Smc1a, Nipbl, Pds5a, Pds5b, and Wapl genes, renowned for their role as cell cycle progression activators, were instead upregulated. Our work suggests that CBN regulates the expression of many genes related to the cell cycle, which are required for axonal maturation, migration, and synaptic plasticity, while not affecting the expression of genes involved in cell death or tumorigenesis.