好氧甲烷氧化细菌(MOB)和亚硝酸盐依赖性厌氧甲烷氧化(n-damo)生物都可能是湿地中重要的甲烷汇。然而,植被类型对湿地有氧MOB和n-damo群落的影响,特别是在人工湿地中,仍然知之甚少。本研究调查了植被类型对城市景观湿地中有氧MOB和n-damo生物的影响。从八个种植不同植物物种的地点收集沉积物。丰度(每克干沉积物1.19-3.27×107个pmoA基因拷贝),丰富度(Chao1估计值=16.3-81.5),多样性(香农指数=2.10-3.15),发现沉积物需氧MOB群落的结构随采样地点的不同而有很大差异。相比之下,n-damo群落丰度(每克干沉积物8.74×105-4.80×106NC1016SrRNA基因拷贝)随采样地点略有变化。丰富度(Chao1估计量=1-11),多样性(香农指数=0-0.78),基于NC1016SrRNA基因的n-damo群落的结构显示出轻微的位点相关变化,而基于pmoA基因的n-damo群落丰富度的空间变化(Chao1估计量=1-8),多样性(香农指数=0-0.99),和结构相当大。植被类型可能对湿地有氧MOB群落产生深远的影响,并且对基于pmoA的n-damo群落的影响比对城市湿地中基于NC1016S的n-damo群落的影响更大。此外,有氧MOB群落比n-damo群落具有更大的丰度和更高的丰富度和多样性。Methylocystis(II型MOB)在城市湿地中占主导地位,而没有检测到已知的I型MOB物种。此外,总有机碳与总氮(C/N)之比可能是沉积物n-damo群落多样性和需氧MOB丰富度的决定因素。
Both aerobic methane-oxidizing bacteria (MOB) and nitrite-dependent anaerobic methane oxidation (n-damo) organisms can be important methane sinks in a wetland. However, the influences of the vegetation type on aerobic MOB and n-damo communities in wetland, especially in constructed wetland, remain poorly understood. The present study investigated the influences of the vegetation type on both aerobic MOB and n-damo organisms in a constructed urban landscape wetland. Sediments were collected from eight sites vegetated with different plant species. The abundance (1.19-3.27 × 107 pmoA gene copies per gram dry sediment), richness (Chao1 estimator = 16.3-81.5), diversity (Shannon index = 2.10-3.15), and structure of the sediment aerobic MOB community were found to vary considerably with sampling site. In contrast, n-damo community abundance (8.74 × 105-4.80 × 106 NC10 16S rRNA gene copies per gram dry sediment) changed slightly with the sampling site. The richness (Chao1 estimator = 1-11), diversity (Shannon index = 0-0.78), and structure of the NC10 16S rRNA gene-based n-damo community illustrated slight site-related changes, while the spatial changes of the pmoA gene-based n-damo community richness (Chao1 estimator = 1-8), diversity (Shannon index = 0-0.99), and structure were considerable. The vegetation type could have a profound impact on the wetland aerobic MOB community and had a stronger influence on the pmoA-based n-damo community than on the NC10 16S-based one in urban wetland. Moreover, the aerobic MOB community had greater abundance and higher richness and diversity than the n-damo community. Methylocystis (type II MOB) predominated in urban wetland, while no known type I MOB species was detected. In addition, the ratio of total organic carbon to total nitrogen (C/N) might be a determinant of sediment n-damo community diversity and aerobic MOB richness.