■亚洲柑橘木虱(ACP,柑橘沉香)是“亚洲念珠菌”(CLas)的重要传播媒介,黄龙病的病原体(HLB),世界上最具破坏性的柑橘病。由于目前没有抗HLB砧木或品种,控制ACP是预防HLB的重要途径。昆虫载体的一些病毒可以用作基因工程材料来控制昆虫载体。
■为了获得有关中国ACP病毒的知识,通过优化逆转录聚合酶链反应(RT-PCR),成功确定了5种RNA和DNA病毒在成人ACP个体中的流行率.5种ACP相关病毒的鉴定如下:通过我们实验室的高通量测序新鉴定出的黄芩病毒2,黄鱼呼肠孤病毒(DcRV),黄参类病毒(DcPLV),柑桔黄片病毒(DcBV),和柑橘黄鱼类病毒(DcDV)。
■DcPLV是最普遍和广泛的ACP相关病毒,其次是DcBV,在所有测试样本的50%以上都检测到了。DcPLV也被证明垂直传播,并且在不同组织的唾液腺中发现更多。所有成年昆虫样本中约有60%与一种以上的昆虫病原体共感染,包括五种ACP相关病毒和CLas。
■这是这些病毒第一次,包括新发现的ACP相关病毒,在中国五个主要柑橘生产省份的自然种群中,已检测到个体成年ACP。这些结果为ACP相关病毒在中国的流行提供了有价值的信息,其中一些有可能被用作生物防治剂。此外,分析单个昆虫媒介中病原体流行的变化是理解CLas之间相互作用的基础,ACP,和昆虫病毒。
UNASSIGNED: Asian citrus psyllid (ACP, Diaphorina citri) is an important transmission vector of \"Candidatus Liberibacter asiaticus\" (CLas), the causal agent of Huanglongbing (HLB), the most destructive citrus disease in the world. As there are currently no HLB-resistant rootstocks or varieties, the control of ACP is an important way to prevent HLB. Some viruses of insect vectors can be used as genetically engineered materials to control insect vectors.
UNASSIGNED: To gain knowledge on viruses in ACP in China, the prevalence of five RNA and DNA viruses was successfully determined by optimizing reverse transcription polymerase chain reaction (RT-PCR) in individual adult ACPs. The five ACP-associated viruses were identified as follows: diaphorina citri bunyavirus 2, which was newly identified by high-throughput sequencing in our lab, diaphorina citri reovirus (DcRV), diaphorina citri picorna-like virus (DcPLV), diaphorina citri bunyavirus (DcBV), and diaphorina citri densovirus-like virus (DcDV).
UNASSIGNED: DcPLV was the most prevalent and widespread ACP-associated virus, followed by DcBV, and it was detected in more than 50% of all samples tested. DcPLV was also demonstrated to propagate vertically and found more in salivary glands among different tissues. Approximately 60% of all adult insect samples were co-infected with more than one insect pathogen, including the five ACP-associated viruses and CLas.
UNASSIGNED: This is the first time these viruses, including the newly identified ACP-associated virus, have been detected in individual adult ACPs from natural populations in China\'s five major citrus-producing provinces. These results provide valuable information about the prevalence of ACP-associated viruses in China, some of which have the potential to be used as biocontrol agents. In addition, analysis of the change in prevalence of pathogens in a single insect vector is the basis for understanding the interactions between CLas, ACP, and insect viruses.