背景:近年来,非小细胞肺癌(NSCLC)的治疗有了很大的改善。然而,无创性早期筛查癌变和进展不清楚。这项研究的目的是探讨外周血免疫细胞在未经治疗的NSCLC患者中的预测价值。
方法:我们回顾性纳入了2016年2月至2019年8月在北京协和医院接受治疗的305例未经治疗的NSCLC患者和132例健康受试者。采用流式细胞术和血常规检测免疫细胞水平。
结果:NSCLC患者的T淋巴细胞水平较低,NK细胞,CD8+T细胞,幼稚CD4+/CD4+,幼稚CD4+T细胞和更高水平的CD4+T细胞,记忆CD4+/CD4+T细胞,记忆CD4+T细胞,CD4+CD28+/CD4+T细胞,CD4+CD28+T细胞,CD8+CD28+/CD8+T细胞,CD8+HLA-DR+/CD8+T细胞,CD8+HLA-DR+T细胞T细胞,CD8+CD38+/CD8+T细胞,CD8+CD38+T细胞和CD4+/CD8+T细胞比对照组。癌症患者与健康个体的特定淋巴细胞亚型的百分比显着不同。例如,癌症患者的B细胞水平较低,CD4+T细胞,幼稚CD4+/CD4+T细胞,幼稚CD4+T细胞,CD4+CD28+T细胞,CD8+CD28+T细胞和更高水平的NK细胞,白细胞(WBC),单核细胞,中性粒细胞,嗜酸性粒细胞,嗜碱性粒细胞,单核细胞与淋巴细胞比率(MLR),中性粒细胞与淋巴细胞比率(NLR),嗜酸性粒细胞与淋巴细胞比率(ELR),嗜碱性粒细胞与淋巴细胞比率(BLR),和血小板与淋巴细胞比率(PLR)。
结论:异常T细胞水平可作为非侵入性早期筛查NSCLC发生和进展的独立预测生物标志物。
BACKGROUND: Treatment for non-small cell lung cancer (NSCLC) has greatly improved in recent years. However, noninvasive early screening for carcinogenesis and progression unclear. The aim of this study was to explore the predictive value of peripheral blood immune cells in untreated NSCLC patients.
METHODS: We retrospectively enrolled 305 untreated NSCLC patients and 132 healthy participants from February 2016 to August 2019 in Peking Union Medical College Hospital. Immune cell levels were determined by flow cytometry and routine blood tests.
RESULTS: NSCLC patients had lower levels of T lymphocytes, NK cells, CD8+ T cells, naïve CD4+/CD4+, naïve CD4+ T cells and higher levels of CD4+ T cells, memory CD4+/CD4+ T cells, memory CD4+ T cells, CD4+CD28+/CD4+ T cells, CD4+CD28+ T cells, CD8+CD28+/CD8+ T cells, CD8+HLA-DR+/CD8+ T cells, CD8+HLA-DR+ T cells T cells, CD8+CD38+/CD8+ T cells, CD8+CD38+ T cells and CD4+/CD8+ T cells than those in controls. The percentages of specific lymphocyte subtypes were significantly different in cancer patients versus healthy individuals. For instance, cancer patients had lower levels of B cells, CD4+ T cells, naïve CD4+/CD4+ T cells, naïve CD4+ T cells, CD4+CD28+ T cells, CD8+CD28+ T cells and higher levels of NK cells, white blood cells (WBC), monocytes, neutrophils, eosinophils, basophils, monocytes to lymphocyte ratio (MLR), neutrophils to lymphocyte ratio (NLR), eosinophil to lymphocyte ratio (ELR), basophil to lymphocyte ratio (BLR), and blood platelet to lymphocyte ratio (PLR).
CONCLUSIONS: Abnormal T cell levels can be used as an independent predictive biomarker for noninvasive early screening in NSCLC occurrence and progression.