目的:通过手术切除和辅助治疗的原发性肿瘤治疗已被广泛研究,但缺乏有效的治疗肿瘤转移的策略和药物。这里,我们描述了一种基于化合物组合的功能产品,它可以用作辅助治疗,并且具有众所周知的抑制癌症转移的机制,改善抗癌治疗,增强免疫力和抗氧化能力。我们设计的组合,名为MVBL,由四种廉价化合物组成:L-硒-甲基硒代半胱氨酸(MSC),D-α-生育酚琥珀酸(VES),β-胡萝卜素(β-Ca),和L-赖氨酸(Lys)。
方法:MVBL对细胞活力的影响,细胞周期,细胞凋亡,细胞迁移,细胞入侵,活性氧(ROS),和紫杉醇(PTX)联合治疗的体外研究。抑制肿瘤转移,抗氧化,并在体内测定了MVBL的免疫增强能力。
结果:MVBL对肿瘤细胞的毒性高于正常细胞。它没有显著影响癌细胞的细胞周期,但增加了它们的凋亡。伤口愈合,附着力,transwell实验表明MVBL显著抑制肿瘤细胞迁移,附着力,和入侵。MVBL致敏MDA-MB-231乳腺癌细胞对PTX,表明它可以作为辅助药物来增强化疗药物的治疗效果。在老鼠身上,实验数据表明,MVBL抑制肿瘤转移,延长了他们的生存时间,并增强了它们的抗氧化能力和免疫功能。
结论:本研究揭示了MVBL在提高免疫力和抗氧化能力方面的作用。防止肿瘤生长,并抑制体内外转移。MVBL可用作癌症治疗中的辅助药物,以改善癌症患者的生存和生活质量。
OBJECTIVE: Primary tumor treatment through surgical resection and adjuvant therapy has been extensively studied, but there is a lack of effective strategies and drugs for the treatment of tumor metastases. Here, we describe a functional product based on a combination of compounds, which can be used as an adjuvant therapy and has well-known mechanisms for inhibiting cancer metastases, improving anti-cancer treatment, and enhancing immunity and antioxidant capacity. Our designed combination, named MVBL, consists of four inexpensive compounds: L-selenium-methylselenocysteine (MSC), D-α-tocopheryl succinic acid (VES), β-carotene (β-Ca), and L-lysine (Lys).
METHODS: The effects of MVBL on cell viability, cell cycle, cell apoptosis, cell migration, cell invasion, reactive oxygen species (ROS), and paclitaxel (PTX)-combined treatment were studied in vitro. The inhibition of tumor metastasis, antioxidation, and immune enhancement capacity of MVBL were determined in vivo.
RESULTS: MVBL exhibited higher toxicity to tumor cells than to normal cells. It did not significantly affect the cell cycle of cancer cells, but increased their apoptosis. Wound healing, adhesion, and transwell assays showed that MVBL significantly inhibited tumor cell migration, adhesion, and invasion. MVBL sensitized MDA-MB-231 breast cancer cells to PTX, indicating that it can be used as an adjuvant to enhance the therapeutic effect of chemotherapy drugs. In mice, experimental data showed that MVBL inhibited tumor metastasis, prolonged their survival time, and enhanced their antioxidant capacity and immune function.
CONCLUSIONS: This study revealed the roles of MVBL in improving immunity and antioxidation, preventing tumor growth, and inhibiting metastasis in vitro and in vivo. MVBL may be used as an adjuvant drug in cancer therapy for improving the survival and quality of life of cancer patients.