■将不同聚合物的优点最大化的复合支架广泛用于引导组织再生(GTR)。一些研究发现,由电纺聚己内酯/氟磷灰石(ePCL/FA)组成的新型复合支架在体外积极促进各种细胞类型的成骨矿化。然而,只有少数研究涉及这种复合支架膜材料在体内的应用。在这项研究中,初步探讨了ePCL/FA复合支架在体内的能力及其可能的作用机制。
■在这项研究中,对ePCL/FA复合支架进行了表征,并研究了其对大鼠骨组织工程和颅骨缺损修复的影响。16只雄性SD大鼠随机分为四组:正常组(整体颅骨结构无缺损),对照组(颅骨缺损),ePCL组(电纺聚己内酯支架修复颅骨缺损),和ePCL/FA组(氟磷灰石修饰的电纺聚己内酯支架修复颅骨缺损)。在1周,2个月,4个月,进行显微计算机断层扫描(micro-CT)分析以比较骨矿物质密度(BMD),骨体积(BV),组织体积(TV),和骨体积百分比(BV/TV)。通过组织学检查(苏木精和伊红,VanGieson,和Masson)分别在4个月时。
■在水接触角测量中,ePCL/FA组的平均接触角明显低于ePCL组,表明FA晶体改善了共聚物的亲水性。Micro-CT分析显示颅骨缺损在1周时无明显变化;BMD,BV,ePCL/FA组的BV/TV在2个月和4个月时明显高于对照组。组织学检查表明,与对照组和ePCL组相比,ePCL/FA复合支架在4个月时几乎完全修复了颅骨缺损。
■生物相容性FA晶体的引入改善了ePCL/FA复合支架的物理和生物学性能;因此,这些支架在骨和骨科再生应用中表现出突出的成骨潜力。
UNASSIGNED: Composite scaffolds that maximize the advantages of different polymers are widely utilized in guided tissue regeneration (GTR). Some studies found that novel composite scaffolds composed of electrospun polycaprolactone/fluorapatite (ePCL/FA) actively promoted the osteogenic mineralization of various cell types in vitro. However, only a few studies have addressed the application of this composite scaffold membrane material in vivo. In this study, the ability of ePCL/FA composite scaffolds in vivo and their possible mechanisms were preliminarily explored.
UNASSIGNED: In this study, ePCL/FA composite scaffolds were characterized and their effects on bone tissue engineering and repair of calvarial defects in rats were examined. Sixteen male Sprague-Dawley (SD) rats were randomly categorized into four groups: normal group (integral cranial structure without defect), control group (cranial defect), ePCL group (cranial defect repaired by electrospun polycaprolactone scaffolds), and ePCL/FA group (cranial defect repaired by fluorapatite-modified electrospun polycaprolactone scaffolds). At 1 week, 2 months, and 4 months, micro-computed tomography (micro-CT) analysis was performed to compare the bone mineral density (BMD), bone volume (BV), tissue volume (TV), and bone volume percentage (BV/TV). The effects of bone tissue engineering and repair were observed by histological examination (hematoxylin and eosin, Van Gieson, and Masson respectively) at 4 months.
UNASSIGNED: In water contact angle measurement, the average contact angle for the ePCL/FA group was significantly lower than that for the ePCL group, indicating that the FA crystal improved the hydrophilicity of the copolymer. Micro-CT analysis revealed that the cranial defect had no significant change at 1 week; however, the BMD, BV, and BV/TV of the ePCL/FA group were significantly higher than those of the control group at 2 and 4 months. Histological examination showed that the cranial defects were almost completely repaired by the ePCL/FA composite scaffolds at 4 months compared to the control and ePCL groups.
UNASSIGNED: The introduction of a biocompatible FA crystal improved the physical and biological properties of the ePCL/FA composite scaffolds; thus, these scaffolds demonstrate outstanding osteogenic potential for bone and orthopedic regenerative applications.