■最近强调了短期健康生活方式计划(LSP)如何改善长期护理(LTC)设施的老年人的功能结果。尽管众所周知,基于生活方式的医学干预措施可以通过调节氧化应激和线粒体功能发挥抗衰老作用,上述影响的潜在机制尚未明确,yet.出于这个原因,在这项研究中,研究的重点是研究老年人短期LSP获益的可能机制.这是通过检查氧化应激和免疫衰老的循环标志物来实现的,如强压素β(Tβ4),LSP前后以及经历或未经历LSP的老年人血浆对内皮细胞的影响。
■54名老年人分为两组(每组n=27):接受LSP的受试者和未接受LSP的受试者(对照)。LSP由热量限制的组合组成,身体活动,心理干预,历时3个月。在LSP之前(T0)和之后(T1)采集血浆样品,并用于测量硫代巴比妥酸反应性物质(TBARS),8-羟基-2-脱氧鸟苷(8OHdG),8-异前列腺素(IsoP),谷胱甘肽(GSH),超氧化物歧化酶(SOD)活性和Tβ4。此外,血浆用于刺激人血管内皮细胞(HUVEC),检查了细胞活力,线粒体膜电位,活性氧(ROS)和线粒体ROS(MitoROS)释放。
■在T1时,在LSP组中我们没有检测到血浆TBARS和IsoP的增加,在对照中观察到。此外,LSP组血浆8OHdG水平低于对照组。此外,LSP组仅显示血浆GSH和SOD活性升高。此外,LSP组血浆Tβ4水平较好。最后,在T1时,与T0相比,在用LSP组血浆处理的HUVEC中,我们发现线粒体膜电位增加,ROS和MitoROS释放减少。
■这项研究的结果表明,老年人体内的短LSP也通过在细胞水平上调节氧化应激而发挥抗衰老作用。这些发现的含义可能与预后和治疗策略有关,可以作为抗衰老方法。
UNASSIGNED: It has recently been highlighted how a short healthy life-style program (LSP) can improve the functional outcomes of older people admitted to a Long-Term Care (LTC) facility. Although it is known that life-style medicine-based interventions can exert anti-aging effects through the modulation of oxidative stress and mitochondrial function, the mechanisms underlying the aforementioned effects have not been clarified, yet. For this reason, in this study, the outcomes were focused on the investigation of the possible mechanisms underlying the benefits of a short LSP in older people. This was achieved by examining circulating markers of oxidative stress and immunosenescence, such as Tymosin β (Tβ4), before and after LSP and the effects of plasma of older people undergone or not LSP on endothelial cells.
UNASSIGNED: Fifty-four older people were divided into two groups (n = 27 each): subjects undergoing LSP and subjects not undergoing LSP (control). The LSP consisted of a combination of caloric restriction, physical activity, and psychological intervention and lasted 3 months. Plasma samples were taken before (T0) and after LSP (T1) and were used to measure thiobarbituric acid reactive substances (TBARS), 8-hydroxy-2-deoxyguanosine (8OHdG), 8-Isoprostanes (IsoP), glutathione (GSH), superoxide dismutase (SOD) activity and Tβ4. In addition, plasma was used to stimulate human vascular endothelial cells (HUVEC), which were examined for cell viability, mitochondrial membrane potential, reactive oxygen species (ROS) and mitochondrial ROS (MitoROS) release.
UNASSIGNED: At T1, in LSP group we did not detect the increase of plasma TBARS and IsoP, which was observed in control. Also, plasma levels of 8OHdG were lower in LSP group vs control. In addition, LSP group only showed an increase of plasma GSH and SOD activity. Moreover, plasma levels of Tβ4 were more preserved in LSP group. Finally, at T1, in HUVEC treated with plasma from LSP group only we found an increase of the mitochondrial membrane potential and a reduction of ROS and MitoROS release in comparison with T0.
UNASSIGNED: The results of this study showed that a short LSP in older persons exerts antiaging effects by modulating oxidative stress also at cellular levels. Implications of those findings could be related to both prognostic and therapeutic strategies, which could be pursued as antiaging methods.