Marmosets通常用于生物医学研究,因此,越来越需要使用大样本量计算更新的参考区间,正确的统计数据,考虑不同的变量。在十年期间(2013-2023年),收集了472种用alphaxalone镇静的健康普通猴的血液学和生化值。比较了假定影响血液参数的变量,即,性别,年龄,住房条件,怀孕,和避孕药具的使用。参考区间是根据观察到的百分位数计算的,没有参数假设,并在Box-Cox变换后进行参数假设。幼猴显示ALP增加,磷酸盐,WBC,淋巴细胞计数,与成年人相比,嗜碱性粒细胞计数和GGT和Fe水平降低。与室外通道的mar猴相比,严格室内的marmosets显示ALT和GGT水平升高,总胆红素和中性粒细胞计数水平降低。怀孕的猿猴显示ALP增加,总胆红素,中性粒细胞计数,单核细胞计数,和嗜碱性粒细胞计数,AST水平降低,ALT,胆固醇,Fe,和淋巴细胞计数与未怀孕的猕猴相比。与未避孕的女性相比,依托孕酮避孕的小鼠的P-LCR降低。更新的参考间隔将帮助研究人员和兽医识别生理和病理变化,以及提高该物种研究的可重复性。
Marmosets are routinely used in biomedical research, therefore there is an increasing need for updated reference intervals calculated using a large sample size, correct statistics, and considering different variables. Hematological and biochemical values from 472 healthy common marmosets sedated with alphaxalone were collected over a ten-year period (2013-2023). The variables assumed to have influenced the blood-based parameters were compared, i.e., sex, age, housing condition, pregnancy, and contraceptive use. Reference intervals were calculated based on observed percentiles without parametric assumptions, and with parametric assumptions following Box-Cox transformation. Juvenile marmosets showed increased ALP, phosphate, WBC, lymphocyte count, and basophil count and decreased levels of GGT and Fe compared to adults. Marmosets housed strictly indoors showed increased ALT and GGT levels and decreased levels of total bilirubin and neutrophil count compared to marmosets housed with outdoor access. Pregnant marmosets showed increased ALP, total bilirubin, neutrophil count, monocyte count, and basophil count, and decreased levels of AST, ALT, cholesterol, Fe, and lymphocyte count compared to non-pregnant marmosets. Etonogestrel contracepted marmosets showed decreased P-LCR compared to females who were not contracepted. Updated reference intervals will aid researchers and veterinarians in identifying physiological and pathological changes, as well as improve the reproducibility of research in this species.