裸花紫珠钩(C.nudiflora)是一种抗炎的,抗菌,抗氧化剂,和止血种族医学。迄今为止,关于裸花梭菌抗溃疡性结肠炎(UC)活性的报道很少。在这项研究中,我们研究了裸花梭菌黄酮提取物对葡聚糖硫酸钠(DSS)诱导的小鼠溃疡性结肠炎的影响。治疗组(CNLFDSS组)和仅药物组(CNLF组)的小鼠分别给予400mg/kg的裸花圆叶黄酮提取物(CNLF),模型组和治疗组给予含2.5%DSS的饮用水。检测到结肠炎的症状,相关指标进行了验证,评估肠屏障功能,并对盲肠的内容物进行肠道微生物分析。结果表明,CNLF能显著缓解小鼠结肠炎的临床症状和组织学形态,抑制促炎因子(TNF-α,IL-6,IL-1β,和IFN-γ),并增加IL-10的水平。NF-κB和MAPK炎症信号通路相关蛋白(p-p65,p-p38,p-ERK,p-JNK)受到调控。紧密连接蛋白(ZO-1,OCLDN,和CLDN1)增加,而D-LA的内容,DAO,LPS降低。此外,16SrRNA测序显示,CNLF恢复了肠道微生物组成,并增加了Prevotellaceae的相对丰度,肠道分枝菌,和Barnesiella_enteutinihominis。总之,CNLF通过抑制炎症水平缓解结肠炎,改善肠屏障的完整性,调节肠道微生物群,因此在UC的治疗中具有很好的应用前景。
Callicarpa nudiflora Hook (C. nudiflora) is an anti-inflammatory, antimicrobial, antioxidant, and hemostatic ethnomedicine. To date, little has been reported regarding the activity of C. nudiflora against ulcerative colitis (UC). In this study, we investigated the effect of a flavonoid extract of C. nudiflora on Dextran Sulfate Sodium (DSS)-induced ulcerative colitis in mice. Mice in the treatment group (CNLF+DSS group) and drug-only (CNLF group) groups were administered 400 mg/kg of flavonoid extract of C. nudiflora leaf (CNLF), and drinking water containing 2.5 % DSS was given to the model and treatment groups. The symptoms of colitis were detected, relevant indicators were verified, intestinal barrier function was assessed, and the contents of the cecum were analyzed for intestinal microorganisms. The results showed that CNLF significantly alleviated the clinical symptoms and histological morphology of colitis in mice, inhibited the increase in pro-inflammatory factors (TNF-α, IL-6, IL-1β, and IFN-γ), and increased the level of IL-10. The expression of NF-κB and MAPK inflammatory signal pathway-related proteins (p-p65, p-p38, p-ERK, p-JNK) was regulated. The expression of tight junction proteins (ZO-1, OCLDN, and CLDN1) was increased, while the content of D-LA, DAO, and LPS was decreased. In addition, 16S rRNA sequencing showed that CNLF restored the gut microbial composition, and increased the relative abundance of Prevotellaceae, Intestinimonas butyriciproducens, and Barnesiella_intestinihominis. In conclusion, CNLF alleviated colitis by suppressing inflammation levels, improving intestinal barrier integrity, and modulating the intestinal microbiota, and therefore has promising future applications in the treatment of UC.