Invasive cervical root resorption (ICRR) is a rare and clinically complex condition marked by the progressive loss of dental hard tissues below the junctional epithelium. This case report outlines the management of a 32-year-old female patient presenting with ICRR class 3 affecting a maxillary incisor. Despite the absence of symptoms, the expansive nature of the defect warranted conservative surgical intervention. The procedure involved the surgical removal of inflamed tissues, followed by an ultraconservative modified pulpotomy utilizing calcium-enriched mixture (CEM) cement through a surgical window. The selected intervention is substantiated by its potential benefits, such as minimal removal of tooth structure and the inherent biocompatibility and sealing capabilities of CEM cement. A one-year follow-up revealed arrested resorption, re-establishment of periodontal attachment, and successful esthetic restoration, affirming the efficacy of vital pulp therapy in surgically addressing advanced ICRR. Accurate diagnosis, strategic treatment planning, and a patient-centered approach proved critical in achieving favorable outcomes.

UNASSIGNED: This non-randomized clinical trial investigated the outcomes of full pulpotomy in adult molars with irreversible pulpitis, comparing those with calcified and non-calcified pulp chambers over 6 and 12 months.
UNASSIGNED: A total of 101 adult permanent molars with irreversible pulpitis, in individuals over 12 years old, were categorized based on pulp chamber calcification observed in radiographic images by two endodontists. Subsequently, full pulpotomy procedures were performed, achieving hemostasis, and applying a 2 mm layer of calcium-enriched mixture (CEM) cement as a pulp covering agent. After 48 hours, the setting of the CEM cement was verified, followed by the application of a layer of resin-modified glass-ionomer. The tooth was then restored using amalgam. Clinical and radiographic evaluations were conducted at 6-month and 1-year follow-ups by blinded endodontists. Success rates were compared using Fisher\'s exact test and logistic regression tests with a significance level of 0.05.
UNASSIGNED: Among the 97 patients with 6-month and 1-year follow-ups, all achieved clinical success. Radiographic success rates were 99% at 6 months and 96.9% at 1 year, regardless of pulp calcification. In the 6-month follow-up, success rates were 98.07% for non-calcified pulp chambers and 100% for calcified pulp chambers. At the 1-year follow-up, success rates were 96.1% and 97.8%, respectively. Statistical analysis showed no significant difference in radiographic success rate between the two groups at both follow-ups (P>0.05).
UNASSIGNED: Full pulpotomy using CEM cement is a successful treatment for adult permanent teeth with calcified and non-calcified pulp chambers presenting signs and symptoms of irreversible pulpitis up to a 1-year follow-up. This study provides compelling evidence that vital pulp therapy can be effectively employed in the pulpotomy of calcified teeth, at least in the short term.

Autogenous tooth transplantation (ATT) is a cost-effective and practical solution for managing severely compromised teeth, provided a suitable donor tooth is available. In this case report, we present a unique and successful ATT procedure performed on a 21-year-old female patient. The patient had an unrestorable adjacent tooth, which was replaced by a fully developed third molar. The procedure involved retrograde root canal filling using a calcium-enriched mixture cement, which took an extraoral time of eight minutes. The second molar was atraumatically extracted, and the mature third molar was immediately transplanted. A one-year clinical examination revealed a symptom-free patient with the transplanted tooth in proper occlusion, fully functional, and without any marginal periodontal issues. Radiographic assessments during follow-up appointments demonstrated bone regeneration, a healthy periodontal ligament, and an absence of external root resorption. This case report highlights the potential of mature third molar ATT combined with retrograde root canal filling as a promising approach to replacing lost permanent molar teeth, ultimately restoring both aesthetics and functionality.

UNASSIGNED: The aims of this in vitro study were to evaluate the effects of two calcium silicate based cements, Calcum-enriched Mixture (CEM) and Biodentine on proliferation of human dental pulp stem cells (hDPSCs) and the effects of proposed cements on the secretion of Transforming Growth Factor β1 (TGF-β1).
UNASSIGNED: The cell cultures of human Dental Pulp Stem Cells (hDPSCs) at passage 3-5 were treated with various dilutions (1/1, 1/2, 1/4, 1/8, 1/16, and 1/32) of CEM and Biodentine extracts to assess the cell proliferation using 3-(4, 5-dimethylthiazol-2-Y1)-2, 5-diphenyltetrazolium brovide (MTT) assay after 48 and 72 h. The amount of TGF-β 1 secretion were estimated after 72 h using an enzyme-linked immunosorbent assay. Data were analyzed using the one-way analysis of variance (ANOVA) followed by the Dunnett\'s test at the level of significance set at 0.05.
UNASSIGNED: CEM showed the highest rates of cell proliferation compared to Biodentine after 72 h (P<0.05). A greater amount of TGF-β1 was secreted by hDPSCs treated with Biodentine compared to CEM (P<0.05). These differences were statistically significant (P<0.05).
UNASSIGNED: In this in vitro study hDPSCs showed more proliferation capacity with CEM rather than Biodentine and TGF-β1 secretion rate in Biodentine was higher.

External inflammatory root resorption (EIRR) is one of the common complications following dental trauma which when remained untreated, may lead to tooth loss. Successful treatment outcomes depend on elimination of bacteria from root canal system and apical sealing. This case presents the endodontic management of an EIRR that was nonresponsive to calcium hydroxide (CH) therapy. An 11-year-old boy was referred for management of a traumatized maxillary central incisor. Tooth #8 was symptom-free, nonresponsive to vitality pulp tests and had an immature root with sever EIRR. Using chemomechanical debridement and CH dressing, the treatment was initiated. The tooth was remained asymptomatic; however, after five weeks the size of periradicular lesion increased and intracanal exudate was present, signifying a resistant endodontic infection. In second appointment, double antibiotic paste (DAP; ciprofloxacin/metronidazole) was applied to the canal. Eight weeks later, the tooth continued to be asymptomatic and the size of the lesion decreased. Finally, the root canal was entirely obturated with calcium-enriched mixture (CEM). At 18-month follow-up, the tooth was asymptomatic/functional, EIRR did not further progress and tooth discoloration was not observed. Based on the results, DAP has the potential to be used to manage the CH-resistant endodontic infection. Furthermore, CEM root filling/sealing seems to be an applicable choice in EIRR management.

UNASSIGNED: Direct pulp capping (DPC) is a conservative vital pulp therapy, which has some limitations in primary dentition. The aim of this study was to evaluate pulpal response of primary teeth after DPC with two biocompatible materials naming calcium-enriched mixture (CEM) and bioactive glass (BAG).
UNASSIGNED: This study was designed as a randomized clinical trial. After obtaining informed consent, 20 sound primary canines scheduled for orthodontic extraction, were selected. Following mechanical pulp exposure, the exposed site was capped with either CEM cement or BAG and then restored with amalgam. Teeth were extracted after two months and examined histopathologically. Parameters of hard tissue bridge (HTB) formation, its type and pulpal inflammation scores, were compared between the two groups. Data were analysed using the Fisher\'s exact test.
UNASSIGNED: All CEM specimens showed inflammation scores of 0 (less than 10%). In the BAG group, inflammation scores of 0, 1 and 2 were observed in 7, 2 and 1 specimens, respectively. Fisher\'s exact test showed no significant differences (P>0.05). All CEM specimens (100%) formed HTB, which was irregular in all cases. In 7 of 10 teeth in BAG, HTB formed and was irregular. Fisher\'s exact test revealed no significant differences between the two groups in this regard (P<0.001).
UNASSIGNED: Both CEM and BAG are suitable DPC agents in terms of HTB formation and pulp inflammation scores.

UNASSIGNED: Coronal restoration could affect the setting reaction of the underlying CEM cement. The aim of the present study was to evaluate the effect of immediate coronal restoration placement on the subsurface microhardness of CEM cement.
UNASSIGNED: In 50 extracted human mandibular molars, access cavities were prepared and CEM cement was placed in the pulp chamber at a 3-mm thickness. Samples were divided into ten groups (n=5). CEM cement was placed and after 10 min, two groups were restored with Zonalin temporary restoration and eight groups were restored with glass ionomer cement (GIC), resin modified glass ionomer (RMGI), resin based composite and amalgam respectively. Vickers microhardness number (VHN) of CEM cement was measured in two time intervals (7- and 21-days). Data was analyzed with SPSS and two-way analysis of variance and Bonferroni tests. Level of significance was set at the 5%.
UNASSIGNED: The mean VHN of CEM cement showed statistically significant differences only between Zonalin and amalgam groups (P=0.021). There were also significant differences considering the effect of time (P=0.042) and material (P=0.046). Although the effect of time-material on the microhardness values showed no statistically significant differences (P=0.636).
UNASSIGNED: Based on the results of the present study, immediate placement of final restorations affects the setting reaction in underlying CEM cement. Therefore, sufficient moist curing and hydration should be guaranteed before placement of the coronal restoration.

UNASSIGNED: This study evaluated the effect of propylene glycol (PG) on the push-out bond strength of calcium-enriched mixture (CEM) cement compared to mineral trioxide aggregate (MTA).
UNASSIGNED: The lumens of two hundred 2±0.2 mm-thick root sections from human extracted teeth were prepared to achieve a diameter of 1.3 mm. The samples were then allocated into eight groups of 25 on the basis of the materials used (MTA or CEM cement) and different proportions of PG (0%, 20%, 505, and 100%). In each group, 0.4 mL of the liquid was mixed with 1 g MTA or CEM cement. After incubation, the push-out strength of the samples was measured using a universal testing machine. Data were analyzed using the two-way ANOVA followed by one-way ANOVA and student\'s t-test.
UNASSIGNED: The MTA group showed significantly higher bond strength in comparison with CEM group (P≤0.001). Also 100% and 20% PG increased the bond strength of MTA (P≤0.001). For CEM cement, 100% and 50% PG decreased the bond strength (P≤0.001).
UNASSIGNED: This in vitro study demonstrated that while PG increased the push-out bond strength of MTA, it is not recommended for mixing with CEM cement.

Background and aims A root perforation is a connection between the root canal system and the external supportive tissues. Strip perforation (SP), occurring within root canals in a strip, can worsen the prognosis of a treated tooth, reduce its mechanical resistance, and impair the tooth structure. One of the suggested methods to treat SP is to seal it with a bio-material such as calcium silicate cement. Therefore, this in vitro study aimed to assess the molar structure impairment due to SP, which requires studying the fracture resistance, and the ability of mineral trioxide aggregate (MTA), bioceramic, and calcium-enriched mixture (CEM) to repair this perforation. Materials and methods Seventy-five molars were instrumented to size #25 and taper 4%, irrigated with sodium hypochlorite and ethylenediaminetetraacetic acid (EDTA), dried, and then divided randomly into five groups (G1-G5): in G1, root canals were filled with gutta-percha and sealer (negative control sample), whereas the rest of the groups (G2-G5) had a manual simulated SP made with Gates Glidden drill at the mesial root of the extracted molar, and filled with gutta-percha and sealer up to their perforation area; in G2, SP was filled with gutta-percha and sealer (positive control sample); G3 used MTA to repair the SP; G4 used bioceramic putty; and G5 used CEM. Fracture resistance tests of the molars were conducted in the crown-apical direction using a universal testing machine. One-way ANOVA test and Bonferroni test were used to study the significance of the differences in the mean values of the tooth fracture resistance, where statistical significance was set at 0.05. Results The ANOVA test showed that there were statistically significant differences between the fracture resistance (in newtons) values among groups (p = 0.000). The Bonferroni test showed that G2 had a smaller fracture resistance mean than the other four study groups (656.53 N; p = 0.000), and that of G5 was smaller than G1, G3, and G4 (794.40 N, 1083.73 N, 1025.20 N, and 1034.20 N, respectively; p = 0.000 in each pairwise comparison). Conclusion SP reduced the fracture resistance of endodontically treated molars. SP restored using MTA and bioceramic putty was better than that treated with CEM and similar to molars without SP. Moreover, MTA and bioceramic putty enhanced the fracture resistance of endodontically treated teeth to levels similar to molars without SP.

UNASSIGNED: The aim of this study was to evaluate the effect of different water-to-powder (WP) proportions on the microhardness and water solubility of calcium-enriched mixture (CEM) cement.
UNASSIGNED: One gram of CEM cement powder was mixed with 0.33 mL, 0.4 mL or 0.5 mL CEM liquid. For water solubility, a total of 60 specimens were prepared (n=20 per each ratio) in the disk-shaped stainless-steel molds with a height of 1.5±0.1 mm and internal diameter of 10.0±0.1 mm. The specimens of each WP ratio were randomly divided into two subgroups: half (n=10) were immersed for one day and the other half (n=10), were kept for 21 days in distilled water. The solubility was calculated as a percentage of the weight loss. To measure microhardness, a total of 30 samples were prepared (10 per each ratio, n=10). The mixtures were transferred to metallic cylindrical molds with internal dimensions of 6±0.1 mm height and 4±0.1 mm diameters. After 4 days the specimens were subjected to Vicker\'s test. The data were analyzed using two-way ANOVA and post-hoc Tukey\'s tests at a significance level of 0.05.
UNASSIGNED: The 0.33 WP ratio showed significantly greater microhardness value (25.98±2.77) compared to 0.4 and 0.5 proportions (P=0.004 and P<0.001 respectively). Significant differences were observed between water solubility values of different WP ratios at both time intervals (P<0.001). At both time intervals, 0.33 and 0.5 WP ratios exhibited the lowest and highest solubility, respectively.
UNASSIGNED: According to the results of this in vitro study, higher WP ratios result in lower microhardness and higher water solubility of the CEM cement. Therefore, the 0.33 WP ratio would be the ideal proportion.