目的:科研界致力于控制激素替代疗法(HRT)的不良影响与绝经后综合征之间的平衡。第三代选择性雌激素受体调节剂和植物雌激素的最新出现为HRT提供了有希望的替代方案。因此,我们评估了Bazedoxifene和Genistein联合应用对实验性卵巢切除术引起的海马神经改变的潜在影响.
方法:为此,我们使用了48只健康的性成熟雌性Wistar大鼠,卵巢切除术(OVX),金雀异黄素治疗的卵巢切除组(OVX+GEN)和Bazedoxifene和金雀异黄素治疗的卵巢切除组(OVX+BZA+GEN)。通过H&E检查来自不同组的海马样本,钙结合蛋白-D28k的银染和免疫组织化学检查,GFAP,和BAX蛋白。我们评估了ERK的海马mRNA表达,CREB,BDNF和TrkB。
结果:我们的组织病理学结果证实,BZA+GEN联合诱导海马神经元结构的恢复,GFAP和BAX平均面积%显著降低,钙结合蛋白-D28k免疫表达显著上调。此外,我们观察到ERK显著上调,CREB,BZA+GEN组与OVX组的BDNF和TrkBmRNA表达比拟。
结论:综合来看,我们的发现提供了一个全面的组织学评估,金雀异黄素和巴多昔芬联合的免疫组织化学和细胞分子基础通过钙结合蛋白的上调对OVX大鼠海马神经改变的改善作用,CERB,BDNF,Trk-B和ERK神经元表达。
The scientific research community devotes stupendous efforts to control the arguable counterbalance between the undesirable effects of hormone replacement therapy (HRT) and post-menopausal syndrome. The recent emergence of 3rd generation selective estrogen receptor modulators and phytoestrogens has provided a promising alternative to HRT. Hence, we assessed the potential effects of combined Bazedoxifene and Genistein on hippocampal neuro-alterations induced by experimental ovariectomy.
For this purpose, we utilized forty-eight healthy sexually mature female Wistar rats assorted to control, ovariectomy (OVX), Genistein-treated ovariectomized (OVX+GEN) and Bazedoxifene and Genistein-treated ovariectomized (OVX+BZA+GEN) groups. Hippocampi samples from various groups were examined by H&E, silver stains and immunohistochemical examination for calbindin-D28k, GFAP, and BAX proteins. We also assessed hippocampal mRNA expression of ERK, CREB, BDNF and TrkB.
Our histopathological results confirmed that combined BZA+GEN induced restoration of hippocampal neuronal architecture, significant reduction of GFAP and BAX mean area % and significant upregulation of calbindin-D28k immunoexpression. Furthermore, we observed significant upregulation of ERK, CREB, BDNF and TrkB mRNA expression in the BZA+GEN group compared to the OVX group.
Taken together, our findings have provided a comprehensive assessment of histological, immunohistochemical and cyto-molecular basis of combined Genistein and Bazedoxifene ameliorative impacts on hippocampal neuro-alterations of OVX rats via upregulation of Calbindin, CERB, BDNF, Trk-B and ERK neuronal expression.