背景:银纳米颗粒(AgNPs)已在植物组织培养中用作生长刺激剂,促进芽的萌生,发芽,和生根。在先前的研究中,使用来自普通β的提取物通过绿色合成来合成和表征AgNPs。cicla(BvAgNP),并验证了它们作为种子消毒剂和抗菌剂的功能。在这项研究中,我们在体外评估了BvAgNP对家蚕和硒菌生长发育的影响,以及乙二醛酶基因的表达。
方法:用25、50和100mg/L的BvAgNP处理体外来自家蚕和S.90天后,形态学特征进行了评估,并通过qPCR分析乙二醛酶基因的表达。
结果:所有处理均抑制了家蚕分枝杆菌的生根,并且没有观察到芽的萌生。S、Undatus,在25mg/L的BvAgNP中显示出最大的生根和芽生成响应。扫描电子显微镜(SEM)结果显示,与两种物种的对照相比,用BvAgNP处理的干细胞中的液泡数量更高。乙二醛酶基因在家蚕分枝杆菌中的表达在所有治疗中都增加,而对于S.Undatus来说,它下降了,然而,在根上增加。
结论:本研究介绍了BvAgNP对家蚕和黑斑链球菌生长和发育的影响,目的是提出促进体外生根和芽萌发的治疗方法。
BACKGROUND: Silver nanoparticles (AgNPs) have been used in plant tissue culture as growth stimulants, promoting bud initiation, germination, and rooting. In prior studies, AgNPs were synthesized and characterized by green synthesis using extracts from Beta vulgaris var. cicla (BvAgNP), and their functionality as seed disinfectant and antimicrobial was verified. In this study, we evaluated the effect of BvAgNP on the growth and development of Mammillaria bombycina and Selenicereus undatus in vitro, as well as the expression of glyoxalase genes.
METHODS: Explants from M. bombycina and S. undatus in vitro were treated with 25, 50, and 100 mg/L of BvAgNP. After 90 days, morphological characteristics were evaluated, and the expression of glyoxalase genes was analyzed by qPCR.
RESULTS: All treatments inhibited rooting for M. bombycina and no bud initiation was observed. S. undatus, showed a maximum response in rooting and bud generation at 25 mg/L of BvAgNP. Scanning electron microscopy (SEM) results exhibited a higher number of vacuoles in stem cells treated with BvAgNP compared to the control for both species. Expression of glyoxalase genes in M. bombycina increased in all treatments, whereas it decreased for S. undatus, however, increasing in roots.
CONCLUSIONS: This study presents the effects of BvAgNP on the growth and development of M. bombycina and S. undatus, with the aim of proposing treatments that promote in vitro rooting and bud initiation.