■结肠癌,排名第四的全球癌症死亡原因,表现出复杂的进展,以遗传变异为标志。在过去的十年里,不同CRISPR系统的应用加速了对结直肠癌(CRC)治疗的研究.
■CRISPR/Cas9,这项研究的关键人物,发现新的致癌基因,肿瘤抑制基因(TSG),和耐药基因。此外,它促进了实验模型的构建,进行全基因组文库筛选,开发新的治疗靶点,特别是体内靶向敲除或分子靶向药物递送,有助于个性化治疗,并显着增强结肠癌患者的护理。在这次审查中,我们提供了对CRISPR/Cas9系统机制的见解,全面探索其在CRC中的应用,跨越筛选,建模,基因功能,诊断,和基因治疗。在承认其变革潜力的同时,这篇文章强调了CRISPR系统的挑战和局限性。
CRISPR/Cas9在CRC研究中的应用为个性化治疗提供了有希望的途径。它具有识别关键基因并启用实验模型和全基因组筛查的潜力,可增强患者护理。这篇综述强调了CRISPR-Cas9基因编辑技术在基础研究中的重要性,诊断,以及结肠癌的治疗前景。
UNASSIGNED: Colon cancer, ranked as the fourth leading global cause of cancer death, exhibits a complex progression marked by genetic variations. Over the past decade, the utilization of diverse CRISPR systems has propelled accelerated research into colorectal cancer (CRC) treatment.
UNASSIGNED: CRISPR/Cas9, a key player in this research, identifies new oncogenes, tumor suppressor genes (TSGs), and drug-resistance genes. Additionally, it facilitates the construction of experimental models, conducts genome-wide library screening, and develops new therapeutic targets, especially for targeted knockout in vivo or molecular targeted drug delivery, contributing to personalized treatments and significantly enhancing the care of colon cancer patients. In this review, we provide insights into the mechanism of the CRISPR/Cas9 system, offering a comprehensive exploration of its applications in CRC, spanning screening, modeling, gene functions, diagnosis, and gene therapy. While acknowledging its transformative potential, the article highlights the challenges and limitations of CRISPR systems.
UNASSIGNED: The application of CRISPR/Cas9 in CRC research provides a promising avenue for personalized treatments. Its potential for identifying key genes and enabling experimental models and genome-wide screening enhances patient care. This review underscores the significance of CRISPR-Cas9 gene editing technology across basic research, diagnosis, and the treatment landscape of colon cancer.