背景:河北,人类免疫缺陷病毒(HIV)患病率较低的省份,也是HIV-1遗传多样性最丰富的地区。HIV-1重组形式一直是影响HIV-1控制和治疗有效性的关键因素。
目的:我们旨在研究新的HIV-1第二代重组形式的亚型间重组结构。
方法:通过系统发育和重组断点分析监测HIV-1亚型是男男性行为者(MSM)中最常见的两种方法。这里,从石家庄市HIV-1血清阳性MSM中获得了三个近全长基因组(NFLGs),中国,他们在2021年从未接受过抗逆转录病毒治疗。
结果:系统发育分析表明,三个NFLG是CRF07_BC和CRF01_AE之间的新型亚型间重组形式。对于NFLG21S009,将四个CRF07_BC基因片段插入到pol中,vif-vpr,vpu-env,和CRF01_AE主链内的nef-3'LTR基因区域,分别。对于NFLG21S095,在HIV-1pol和vpu区域中鉴定了四个断点。NFLG21S370在HIV-1pol和vpu-env基因区域中包含四个基因重组断点。在这三个NFLG中,NFLG21S009包含最多的断点,分布在pol,vif,vpr,vpu,env,和nef地区,分别。在gag-pol地区,三个NFLG只有一个CRF07_BC基因片段插入到4250和4792之间的基因点。
结论:我们的研究结果提供了强有力的证据,表明新的重组形式的监测对于更好地控制HIV是必要的。
Hebei, a province with a low Human Immunodeficiency Virus (HIV) prevalence, is also a region with the most abundant HIV-1 genetic diversity. HIV-1 recombinant forms have been the key factor influencing the effectiveness of HIV-1 control and therapy.
We aimed to study inter-subtype recombinant structures of new HIV-1-second generation recombinant forms.
Monitoring the HIV-1 subtype by phylogenetic and recombinant breakpoint analyses are the two most frequent methods among men who have sex with men (MSM). Here, three near full-length genomes (NFLGs) were obtained from HIV-1 seropositive MSM in Shijiazhuang City, China, who have never received antiretroviral therapy in 2021.
Phylogenetic analysis indicated that three NFLGs were novel inter-subtype recombinant forms between CRF07_BC and
CRF01_AE. For the NFLG 21S009, four CRF07_BC gene fragments were inserted into the pol, vif-vpr, vpu-env, and nef-3` LTR gene regions within a CRF01_ AE backbone, respectively. For the NFLG 21S095, four breakpoints were identified in HIV-1 pol and vpu regions. The NFLG 21S370 contained four gene recombinant breakpoints within HIV-1 pol and vpu-env gene regions. Of these three NFLGs, the NFLG 21S009 contained the most breakpoints, distributed in the pol, vif, vpr, vpu, env, and nef regions, respectively. In the gag-pol regions, three NFLGs had only one CRF07_BC gene fragment inserted into gene points between 4250 and 4792.
Our findings provide strong evidence that the surveillance of novel recombinant forms is necessary for the increase in better control of HIV.