目的:蚊子是携带可影响人类和动物的致病因子的重要媒介。DNA条形码是一种互补的鉴定,可用于验证蚊子的形态特征。本研究的目的是确定COI基因的线粒体序列,并根据所研究的蚊子物种的遗传差异构建分子系统发育。
方法:在本研究中,在NayPyiTaw地区收集的混合蚊子样品上进行DNA提取和线粒体细胞色素氧化酶亚基I基因(COI)的扩增,缅甸。
结果:COI基因的片段与埃及伊蚊的序列具有99-100%的同一性,阿尔米格雷斯亚阿尔巴图斯,淡色库蚊复合体,Cx.Quinquefasciatus,分别,存入GenBank。该研究对来自每个Ar的两个单倍型进行了分类。下半叶和Cx。Pipiens复合物COI基因序列,以及来自Cx的三种单倍型。quinquefasciatusCOI基因序列。在Ar中观察到最高的单倍型多样性和核苷酸多样性。亚耳目种群(Hd=1.0000;π=0.0033),其次是Cx。pipiens复合体和Cx。quinquefasciatus种群。
结论:这项研究为具有医学和兽医学意义的蚊子载体的分子鉴定和遗传多样性提供了有用的信息,这可能有助于改善蚊子控制计划。
OBJECTIVE: Mosquitoes are important vectors that carry disease-causing agents that can affect humans and animals. DNA barcoding is a complementary identification which can be used to validate morphological characterization of mosquito species. The objectives of this study were to identify the mitochondrial sequence of the COI gene and to construct a molecular phylogeny based on the genetic divergence of the mosquito species studied.
METHODS: In this study, DNA extraction and the amplification of the mitochondrial cytochrome oxidase subunit I genes (COI) were performed on pooled mosquito samples collected in Nay Pyi Taw area, Myanmar.
RESULTS: Fragments of the COI gene showed 99-100% identity with sequences of Aedes aegypti, Armigeres subalbatus, Culex pipiens complex, and Cx. quinquefasciatus, respectively, deposited in GenBank. This study categorized two haplotypes from each Ar. subalbatus and Cx. pipiens complex COI gene sequence, as well as three haplotypes from Cx. quinquefasciatus COI gene sequences. The highest haplotype diversity and nucleotide diversity were observed in the Ar. subalbatus population (Hd = 1.0000; π = 0.0033), followed by the Cx. pipiens complex and Cx. quinquefasciatus populations.
CONCLUSIONS: This study provides useful information on the molecular identification and genetic diversity of mosquito vectors with medical and veterinary significance, which may assist in the improvement of mosquito control programs.