陆地生态系统中的微塑料(MPs)积累可以通过改变微生物和土壤结构来影响温室气体(GHG)的产生。目前,关于MPs对植物影响的研究并不一致,与温室气体相关的潜在分子机制尚不清楚。第一次,我们进行了一项微观研究,以探讨MP添加的影响(Rawvs.老年人)和长臂木霉和枯草芽孢杆菌接种(唯一与组合)温室气体排放,土壤群落结构,理化性质,和酶活性。我们的结果表明,添加老化的MPs显著增加了温室气体排放(N2O(+16%)和CO2(+21%),分别),C和N循环基因表达,微生物生物质碳,和土壤理化性质比原始MPs。然而,土壤微生物群落结构和酶活性在原始MPs添加处理中得到增强,无论添加到土壤中的MPs类型如何。然而,通过改变两种类型的MPs添加处理中C和N循环基因的表达,微生物接种显着减少了GHGs的排放。土壤微生物群落结构,酶活性,在这两种类型的MPs中,在存在微生物接种的情况下,理化性质和微生物生物量碳都得到了增强。在木霉属和枯草芽孢杆菌的单独和联合接种中,共同施用的木霉和枯草芽孢杆菌大大减少了温室气体排放(N2O(-64%)和CO2(-61%),分别)通过改变C和N循环基因的表达而与使用的MPs类型无关。联合接种还增强了土壤酶活性,微生物群落结构,这两种类型的MPs处理的理化性质和微生物生物量碳。我们的发现提供了证据,表明聚乙烯MPs可能会造成温室气体排放的高风险,而木霉属和枯草芽孢杆菌的联合应用通过改变C和N循环基因表达显着减少了温室气体排放。土壤微生物群落结构,和陆地生态系统中MPs污染下的酶活性。
Microplastics (MPs) accumulation in terrestrial ecosystems can affect greenhouse gases (GHGs) production by altering microbial and soil structure. Presently, research on the MPs effect on plants is not consistent, and underlying molecular mechanisms associated with GHGs are yet unknown. For the first time, we conducted a microcosm study to explore the impact of MPs addition (Raw vs. aged) and Trichoderma longibrachiatum and Bacillus subtilis inoculation (Sole vs. combination) on GHGs emission, soil community structure, physiochemical properties, and enzyme activities. Our results indicated that the addition of aged MPs considerably enhanced the GHGs emissions (N2O (+16%) and CO2 (+21%), respectively), C and N cycling gene expression, microbial biomass carbon, and soil physiochemical properties than raw MPs. However, the soil microbial community structure and enzyme activities were enhanced in raw MPs added treatments, irrespective of the MPs type added to soil. However, microbial inoculation significantly reduced GHGs emission by altering the expression of C and N cycling genes in both types of MPs added treatments. The soil microbial community structure, enzymes activities, physiochemical properties and microbial biomass carbon were enhanced in the presence of microbial inoculation in both type of MPs. Among sole and combined inoculation of Trichoderma and Bacillus subtilis, the co-applied Trichoderma and Bacillus subtilis considerably reduced the GHGs emission (N2O (-64%) and CO2 (-61%), respectively) by altering the expression of C and N cycling genes regardless of MPs type used. The combined inoculation also enhanced soil enzyme activities, microbial community structure, physiochemical properties and microbial biomass carbon in both types of MPs treatment. Our findings provide evidence that polyethylene MPs likely pose a high risk of GHGs emission while combined application of Trichoderma and Bacillus subtilis significantly reduced GHGs emission by altering C and N cycling gene expression, soil microbial community structure, and enzyme activities under MPs pollution in a terrestrial ecosystem.