■长链非编码RNA(lncRNA)作为竞争性内源性RNA(ceRNA)在肿瘤进展中发挥关键作用。然而,肺腺癌(LUAD)中与预后相关的ceRNA网络尚未得到很好的表征。
■LUAD数据集从TCGA数据库下载,将患者分为转移组和非转移组。lncRNAs(DELs)的差异表达,miRNA(DEM),使用Limma软件包分析mRNA(DEG)。接下来,miRNA之间的相互作用,lncRNA,和mRNA通过miRcode预测,miRTar-Base,miRDB,和TargetScan。使用Cytoscape软件基于这些相互作用构建ceRNA网络。通过GO和KEGG进行DEG富集分析。经过预后分析,我们进一步筛选了相关分子并构建了预后相关的ceRNA网络。此外,lncRNA之间的相互作用,miRNA,并通过生物学实验对mRNA进行了验证。
■854个DEL,150DEM,并确定了2211个转移和非转移LUAD患者之间的DEGs。功能富集分析表明,DEGs与LUAD进展中涉及的关键生物过程密切相关。预后相关的ceRNA网络包括1个miRNA,2个lncRNAs,和4个mRNA。在这个网络中,MIR155HG和ADAMTS9-AS2可以作为miR-212的ceRNA来调节EPM2AIP1、LAX1、PRICKLE2和CD226。此外,我们的研究证实,MIR155HG抑制增殖,迁移,和LUAD细胞的侵袭通过海绵miR-212-3p调节CD226。
■该ceRNA网络有助于理解LUAD的发病机制。此外,网络中的分子是LUAD预后的有价值的预测因子以及潜在的治疗性生物标志物.
UNASSIGNED: Long non-coding RNAs (lncRNAs) functioning as competing endogenous RNAs (ceRNAs) play critical roles in tumour progression. However, prognosis-related ceRNA networks in lung adenocarcinoma (LUAD) have not been well characterised.
UNASSIGNED: LUAD datasets were downloaded from the TCGA database, and the patients were divided into metastasis and non-metastasis groups. The differential expression of lncRNAs (DELs), miRNAs (DEMs), and mRNAs (DEGs) was analysed using the Limma package. Next, interactions between miRNA, lncRNA, and mRNA were predicted by miRcode, miRTar-Base, miRDB, and TargetScan. The ceRNA network was constructed based on these interactions using Cytoscape software. DEG enrichment analysis was performed by GO and KEGG. After the prognosis analysis, we further screened molecules and constructed the prognosis-related ceRNA network. Moreover, the interactions between lncRNA, miRNA, and mRNA were validated by biological experiments.
UNASSIGNED: 854 DELs, 150 DEMs, and 2211 DEGs between metastasis and non-metastasis LUAD patients were identified. Functional enrichment analysis suggested that DEGs were closely related to key biological processes involved in LUAD progression. The prognosis-related ceRNA network included 1 miRNA, 2 lncRNAs, and 4 mRNAs. In this network, MIR155HG and ADAMTS9-AS2 can function as ceRNAs of miR-212 to regulate EPM2AIP1, LAX1, PRICKLE2, and
CD226. Moreover, our study confirmed that MIR155HG inhibited the proliferation, migration, and invasion of LUAD cells by sponging miR-212-3p to regulate
CD226.
UNASSIGNED: This ceRNA network contributes to understanding the pathogenesis of LUAD. Furthermore, the molecules in the network are valuable predictive factors for LUAD prognosis as well as potential therapeutic biomarkers.