CO2吸入是目前实验室大鼠和小鼠最常用的安乐死方法,它通常用于临床生化测定的进一步终端血液采样。最近,由于与吸入二氧化碳后发展的某些过程相关的动物福利问题,这种方法受到了批评。应激反应和临床实验室参数的值显著取决于所用的麻醉药,方法,以及采血的部位.特别是在小型啮齿动物中,急性终末状态,然后是一系列代谢反应,这些反应可能会影响所研究的生化特征,并可能导致动物不必要的痛苦。这项研究的目的是比较吸入CO2或肌内注射替利塔明-唑拉西泮-赛拉嗪(TZX)后收集的近交SpragueDawley大鼠和CD-1小鼠血清的生化参数的稳定性。血清总蛋白和白蛋白含量,胆固醇,甘油三酯,天冬氨酸转氨酶(AST),丙氨酸氨基转移酶(ALT),碱性磷酸酶(ALP),总胆红素,与吸入CO2相比,注射TZX降低了肌酐。此外,钙的水平,磷酸盐,TZX降低了氯化物和钾CO2管理,而钠的含量增加。最后,吸入CO2后,大鼠和小鼠的大多数血清临床生化指标水平往往被高估,这可能会掩盖动物试验中抗炎药的可能作用。TZX对小型啮齿动物的注射麻醉是一种更可行的终末采血方法,这也减少了动物的痛苦。
CO2 inhalation is currently the most common method of euthanasia for laboratory rats and mice, and it is often used for further terminal blood sampling for clinical biochemical assays. Lately, this method has been criticized due to animal welfare issues associated with some processes that develop after CO2 inhalation. The stress reaction and the value of the clinical laboratory parameters significantly depend on the used anesthetics, method, and the site of blood sampling. Especially in small rodents, an acute terminal state followed by a cascade of metabolic reactions that can affect the studied biochemical profile may develop and cause unnecessary suffering of animals. The aim of this study was to compare the stability of biochemical parameters of outbred Sprague Dawley rats and CD-1 mice serum collected after CO2 inhalation or the intramuscular injection of tiletamine-zolazepam-xylazine (TZX). The serum content of total protein and albumin, cholesterol, triglycerides, aspartate aminotransferase (AST), alanine aminotr ansferase (ALT), alkaline phosphatase (ALP), total bilirubin, and creatinine was decreased by the injection of TZX in comparison with CO2 inhalation. In addition, the levels of calcium, phosphates, chlorides and potassium were lowered by TZX vs. CO2 administration, while the level of sodium increased. Finally, the level of the majority of serum clinical biochemical parameters in rats and mice tend to be overestimated after CO2 inhalation, which may lead to masking the possible effect of anti-inflammatory drugs in animal tests. Injection anesthesia for small rodents with TZX is a more feasible method for terminal blood sampling, which also reduces the suffering of animals.