骨关节炎是慢性关节疾病的主要原因之一,并可能逐渐导致老年人的残疾。本研究旨在鉴定终末期骨关节炎的差异表达基因和相关信号通路。
■通过GSE32317数据集中的基因表达综合2R(GEO2R)检查了骨关节炎早期和晚期阶段差异表达的信使RNA。随后,基因本体论(GO)富集,京都基因和基因组百科全书(KEGG),并进行了蛋白质-蛋白质相互作用(PPI)分析。此外,使用miRcode数据库研究了靶向hub基因的microRNA。这项研究是在Bam医科大学和Rajaie心血管大学联合进行的,医疗和研究中心,2022年10月。
■差异表达数据表明,在终末期膝骨关节炎中,134个基因下调,189个基因上调。富集和PPI分析的结果确定了4个终末期膝骨性关节炎相关的枢纽基因:IL-1B,CD19,CACNA1G,ALDH18A1膝关节骨性关节炎相关关键基因参与Wnt信号通路,B细胞受体信号,钙信号,昼夜节律夹带,精氨酸和脯氨酸代谢,轴突引导,和细胞因子-细胞因子受体途径。此外,预测了靶向上述4个基因的microRNA.
■本研究首次为关键基因的潜在治疗靶点提供了新的见解,即CD19,CACNA1G,和ALDH18A1,在终末期骨关节炎中差异表达及其相关信号通路和相互作用的microRNAs。
UNASSIGNED: Osteoarthritis is one of the principal causes of chronic joint disease and may progressively engender disability in elderly individuals. The present study aimed to identify differentially expressed genes and associated signaling pathways in end-stage osteoarthritis.
UNASSIGNED: Differentially expressed messenger RNAs in the early and end stages of osteoarthritis were examined through gene expression omnibus 2R (GEO2R) in the GSE32317 dataset. Subsequently, gene ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) analyses were conducted. Furthermore, microRNAs targeting hub genes were investigated using the miRcode database. This study was conducted jointly at Bam University of Medical Sciences and Rajaie Cardiovascular, Medical and Research Center on October 2022.
UNASSIGNED: Differentially expressed data demonstrated downregulation in 134 genes and upregulation in 189 genes in end-stage knee osteoarthritis. The results of the enrichment and PPI analyses determined 4 end-stage knee osteoarthritis-related hub genes: IL-1B, CD19,
CACNA1G, and ALDH18A1. The knee osteoarthritis-related key genes were involved in the Wnt signaling, B cell receptor signaling, calcium signaling, circadian entrainment, arginine and proline metabolism, axon guidance, and cytokine-cytokine receptor pathways. Additionally, the microRNAs targeting the 4 aforementioned genes were predicted.
UNASSIGNED: The present study is the first to provide fresh insights into the potential therapeutic targets of key genes, namely CD19,
CACNA1G, and ALDH18A1, differentially expressed in end-stage osteoarthritis and their relevant signaling pathways and interactive microRNAs.