UNASSIGNED:棕色脂肪组织(BAT)以热量的形式散发热量,并在葡萄糖和脂质代谢中起作用。因此,增强和激活BAT是针对肥胖的新治疗策略的重点,代谢综合征的主要危险因素。维生素D系统在矿物质稳态中起着至关重要的作用,骨代谢,细胞增殖和分化。在这项研究中,我们研究了维生素D3[1,25(OH)2D3]对棕色脂肪细胞分化的影响。
UNASSIGNED:小鼠成纤维样细胞系C3H10T1/2在1,25(OH)2D3存在下分化成棕色脂肪细胞。1,25(OH)2D3对棕色脂肪细胞分化的影响通过测量脂质积累来评估,相关基因的表达,和细胞毒性。使用细胞计数试剂盒-8测定法测量C3H10T1/2细胞的活力。使用定量逆转录-聚合酶链反应研究基因表达。使用蛋白质印迹估计蛋白质表达。
UNASSIGNED:1,25(OH)2D3抑制脂肪细胞分化,并在1nM时发挥细胞毒性作用。然而,在生理浓度范围(50-250μM),1,25(OH)2D3促进解偶联蛋白1(UCP1)在C3H10T1/2细胞中的表达。当分化开始后48小时添加1,25(OH)2D3时,没有观察到这种效果。这表明维生素D系统在分化程序的早期起作用。我们表明,1,25(OH)2D3增加了棕色脂肪生成的两个关键调节因子的表达,含有16(Prdm16)和过氧化物酶体增殖物激活受体γ共激活因子-1α(Pgc1α)的PR结构域。此外,1,25(OH)2D3以剂量依赖性方式增加3T3-L1米色脂肪生成中的Ucp1表达。
UNASSIGNED:这些数据表明维生素D及其类似物作为治疗肥胖和相关代谢疾病的治疗剂的潜力。
UNASSIGNED: Brown adipose tissue (BAT) dissipates caloric energy as heat and plays a role in glucose and lipid metabolism. Therefore, augmentation and activation of BAT are the focus of new treatment strategies against obesity, a primary risk factor of metabolic syndrome. The vitamin D system plays a crucial role in mineral homeostasis, bone metabolism, and cell proliferation and differentiation. In this study, we investigated the effects of vitamin D3 [1,25(OH)2D3] on brown adipocyte differentiation.
UNASSIGNED: The mouse fibroblast-like cell line C3H10T1/2 was differentiated into brown adipocytes in the presence of 1,25(OH)2D3. The effect of 1,25(OH)2D3 on brown adipocyte differentiation was assessed by measuring lipid accumulation, the expression of related genes, and cytotoxicity. The viability of C3H10T1/2 cells was measured using the Cell Counting Kit-8 assay. Gene expression was investigated using quantitative reverse transcription-polymerase chain reaction. Protein expression was estimated using western blotting.
UNASSIGNED: 1,25(OH)2D3 inhibited adipocyte differentiation and exerted a cytotoxic effect at 1 nM. However, in the physiological concentration range (50-250 pM), 1,25(OH)2D3 promoted uncoupling protein 1 (UCP1) expression in C3H10T1/2 cells. This effect was not observed when 1,25(OH)2D3 was added 48 h after the initiation of differentiation, suggesting that the vitamin D system acts in the early phase of the differentiation program. We showed that 1,25(OH)2D3 increased the expression of two key regulators of brown adipogenesis, PR domain containing 16 (Prdm16) and peroxisome proliferator-activated receptor γ coactivator-1α (Pgc1α ). Furthermore, 1,25(OH)2D3 increased Ucp1 expression in 3T3-L1 beige adipogenesis in a dose-dependent manner.
UNASSIGNED: These data indicate the potential of vitamin D and its analogs as therapeutics for the treatment of obesity and related metabolic diseases.