建议进行冲击氯化和补救冲洗,以解决建筑物或建筑物(重新)调试期间的嗜肺军团菌(Lp)污染。然而,一般微生物测量数据(三磷酸腺苷[ATP],总细胞计数[TCC]),Lp的丰度缺乏支持其临时实施的变化的水需求。在这项研究中,休克氯化(20-25mg/L游离氯,16小时)或补救冲洗(5分钟冲洗)结合不同的冲洗方案(每天,每周,停滞)在两个淋浴系统中重复淋浴头进行了研究。结果表明,停滞和休克氯化的结合促进了生物量的再生,ATP和TCC在第一次抽签中达到4.31-7.07倍和3.51-5.68倍的大再生长因子,分别,从基线值。相反,补救冲洗后停滞通常会导致Lp可培养性和基因拷贝(gc)完全或更大的再生。无论干预如何,每天冲洗淋浴头导致显著(p<0.05)降低ATP和TCC,以及Lp浓度低于每周冲洗,总的来说。尽管如此,Lp的浓度范围为11至223,为每升最可能的数量(MPN/L),并且在补救冲洗后与基线值相同的数量级(103-104gc/L)。尽管每天/每周冲洗,与休克氯化不同,休克氯化抑制了Lp的可培养性(降低3-log)两周,基因拷贝降低了1-log。这项研究提供了有关补救和预防策略的最佳短期组合的见解,在实施适当的工程控制或建筑物范围内的治疗之前,可以考虑这些策略。
Shock chlorination and remedial flushing are suggested to address Legionella pneumophila (Lp) contamination in buildings or during their (re)commissioning. However, data on general microbial measurements (adenosine tri-phosphate [ATP], total cell counts [TCC]), and the abundance of Lp are lacking to support their temporary implementation with variable water demands. In this study, the weekly short-term (3-week) impact of shock chlorination (20-25 mg/L free chlorine, 16 h) or remedial flushing (5-min flush) combined with distinct flushing regimes (daily, weekly, stagnant) was investigated in duplicates of showerheads in two shower systems. Results showed that the combination of stagnation and shock chlorination prompted biomass regrowth, with ATP and TCC in the first draws reaching large regrowth factors of 4.31-7.07-fold and 3.51-5.68-fold, respectively, from baseline values. Contrastingly, remedial flushing followed by stagnation generally resulted in complete or larger regrowth in Lp culturability and gene copies (gc). Irrespective of the intervention, daily flushed showerheads resulted in significantly (p < 0.05) lower ATP and TCC, as well as lower Lp concentrations than weekly flushes, in general. Nonetheless, Lp persisted at concentrations ranging from 11 to 223 as the most probable number per liter (MPN/L) and in the same order of magnitude (103-104 gc/L) than baseline values after remedial flushing, despite daily/weekly flushing, unlike shock chlorination which suppressed Lp culturability (down 3-log) for two weeks and gene copies by 1-log. This study provides insights on the most optimal short-term combination of remedial and preventative strategies that can be considered pending the implementation of suitable engineering controls or building-wide treatment.