This study presents an updated analysis spanning over two decades (1999-2023) of climate, water quality, and operational data from two drinking water facilities in Atlantic Canada that previously experienced gradual increases in the natural organic matter (NOM) concentration and brownification. The goal was to assess the impact of recent extreme weather events on acute NOM concentration increases and drinking water treatment processes. In 2023, a dry spring combined with a warm and wet summer caused NOM in the water supplies to increase by >67% (as measured by color). To mitigate increased NOM concentration, the alum dose nearly doubled in 2023 compared to that in 2022. Disinfection byproducts were elevated following the event but remained within the compliance levels. From 1999 to 2023, the two plants responded to gradual climate change impacts and brownification, with alum dose increases of between 4.1 and 8.3 times. Equivalent CO2 emissions were estimated for alum usage, which increased by 3 to 7-fold in 2023 compared to when the plants were commissioned decades prior. The plants were not only adversely impacted by climate change but also contributed to the global CO2 burden. Thus, a paradigm shift toward sustainable alternatives for NOM removal is required in the water sector, and climate change adaptation and mitigation principles are urgently needed.

Brownification in aquatic ecosystems under global change has attracted attention. The composition and quantity of dissolved organic matter transported from various land use types to lakes differ significantly, causing varying ecological effects of lake brownification by region. Bacterial communities make a significant contribution to the material cycle of ecosystems and are sensitive to environmental changes. In this study, a series of mesocosm systems were used to simulate forest lakes and urban lakes with different degrees of brownification, and a high-throughput amplicon sequencing technique was used to explore the changes in the composition, structure, and function of bacterial communities in shallow lakes undergoing brownification. Principal coordinate analysis (PCoA) and Jensen‒Shannon distance typing analysis both indicated significant differences in bacterial communities between forest lakes and urban lakes. The α diversity of bacterial communities in urban lakes increased with the degree of brownification. However, whether forest lakes or urban lakes, brownification increased the abundance of carbon cycling-related bacterial phyla (Proteobacteria, Poribacteria, and Chloroflexi) and nitrogen cycling-related bacterial genera (Microbacteriaceae, Limnohabitans, Comamonadaceae, Bacillus, and Rhizobiales_Incertae_Sedis). Additionally, the carbon and nitrogen cycling functions of bacterial communities in forest lakes are dominant, while those in urban lakes are dominated by functions related to light. Our study has preliminarily revealed that lake brownification promotes the growth of carbon and nitrogen cycling microorganisms, providing a new paradigm for understanding the response of lake ecosystems in different catchment areas to environmental changes and the carbon and nitrogen cycling processes in shallow lake ecosystems.

Wetland habitats are changing under multiple anthropogenic pressures. Nutrient leakage and pollution modify physico-chemical state of wetlands and affect the ecosystem through bottom-up processes, while alien predators affect the ecosystems in a top-down manner. Boreal wetlands are important breeding areas for several waterbird species, the abundances of which potentially reflect both bottom-up and top-down ecosystem processes. Here, we use long-term national monitoring data gathered from c. 130 waterbird breeding sites in Finland from the 1980s to the 2020s. We hypothesised that the physico-chemical state of the waters and increasing alien predator abundance both play a role in steering the waterbird population trends. We set out to test this hypothesis by relating population changes of 17 waterbird species to changes in water chemistry and to regional alien predator indices while allowing species-specific effects to vary with foraging niche (dabblers, invertivore divers, piscivorous divers, herbivores), nesting site, female mass and habitat (oligotrophic, eutrophic). We found niche and nesting site-specific, habitat-dependent changes in waterbird numbers. While the associations with higher phosphorus levels and browning water were in overall positive at the oligotrophic lakes, the numbers of invertivore and piscivore diving ducks were most strongly negatively associated with higher phosphorus levels and browning water at the eutrophic lakes. Furthermore, increased pH levels benefitted piscivores. Invertivore diving duck species nesting on the wetlands had declined most on sites with high alien predator indices. Large herbivorous species and species preferring oligotrophic lakes seem to be successful. We conclude that the large-scale breeding waterbird decline in Finland is closely connected to both bottom-up and top-down processes, where negative associations are emphasised especially at eutrophic lakes. Niche-, nest site- and habitat-specific management actions are required to conserve declining waterbird populations. Managing wetlands on catchments level together with alien predator control may provide important approaches to future wetland management.

Eutrophication and brownification are ongoing environmental problems affecting aquatic ecosystems. Due to anthropogenic changes, increasing amounts of organic and inorganic compounds are entering aquatic systems from surrounding catchment areas, increasing both nutrients, total organic carbon (TOC), and water color with societal, as well as ecological consequences. Several studies have focused on the ability of wetlands to reduce nutrients, whereas data on their potential to reduce TOC and water color are scarce. Here we evaluate wetlands as a potential multifunctional tool for mitigating both eutrophication and brownification. Therefore, we performed a study for 18 months in nine wetlands allowing us to estimate the reduction in concentrations of total nitrogen (TN), total phosphorus (TP), TOC and water color. We show that wetland reduction efficiency with respect to these variables was generally higher during summer, but many of the wetlands were also efficient during winter. We also show that some, but not all, wetlands have the potential to reduce TOC, water color and nutrients simultaneously. However, the generalist wetlands that reduced all four parameters were less efficient in reducing each of them than the specialist wetlands that only reduced one or two parameters. In a broader context, generalist wetlands have the potential to function as multifunctional tools to mitigate both eutrophication and brownification of aquatic systems. However, further research is needed to assess the design of the generalist wetlands and to investigate the potential of using several specialist wetlands in the same catchment.

Shallow lake ecosystems are particularly prone to disturbances such as pulsed dissolved organic matter (allochthonous-DOM; hereafter allo-DOM) loadings from catchments. However, the effects of allo-DOM with contrasting quality (in addition to quantity) on the planktonic communities of microbial loop are poorly understood. To determine the impact of different qualities of pulsed allo-DOM disturbance on the coupling between bacteria and ciliates, we conducted a mesocosm experiment with two different allo-DOM sources added to mesocosms in a single-pulse disturbance event: Alder tree leaf extract, a more labile (L) source and HuminFeed® (HF), a more recalcitrant source. Allo-DOM sources were used as separate treatments and in combination (HFL) relative to the control without allo-DOM additions (C). Our results indicate that the quality of allo-DOM was a major regulator of planktonic microbial community biomass and/or composition through which both bottom-up and top-down forces were involved. Bacteria biomass showed significant nonlinear responses in L and HFL with initial increases followed by decreases to pre-pulse conditions. Ciliate biomass was significantly higher in L compared to all other treatments. In terms of composition, bacterivore ciliate abundance was significantly higher in both L and HFL treatments, mainly driven by the bacterial biomass increase in the same treatments. GAMM models showed negative interaction between metazoan zooplankton biomass and ciliates, but only in the L treatment, indicating top-down control on ciliates. Ecosystem stability analyses revealed overperformance, high resilience and full recovery of bacteria in the HFL and L treatments, while ciliates showed significant shift in compositional stability in HFL and L with incomplete taxonomic recovery. Our study highlights the importance of allo-DOM quality shaping the response within the microbial loop not only through triggering different scenarios in biomass, but also the community composition, stability, and species interactions (top-down and bottom-up) in bacteria and plankton.

The browning of surface waters due to the increased terrestrial loading of dissolved organic carbon is observed across the northern hemisphere. Brownification is often explained by changes in large-scale anthropogenic pressures (including acidification, and climate and land-use changes). We quantified the effect of environmental changes on the brownification of an important lake for birds, Kukkia in southern Finland. We studied the past trends of organic carbon loading from catchments based on observations taken since the 1990s. We created hindcasting scenarios for deposition, climate and land-use change in order to simulate their quantitative effect on brownification by using process-based models. Changes in forest cuttings were shown to be the primary reason for the brownification. According to the simulations, a decrease in deposition has resulted in a slightly lower leaching of total organic carbon (TOC). In addition, runoff and TOC leaching from terrestrial areas to the lake was smaller than it would have been without the observed increasing trend in temperature by 2 °C in 25 years.

Browning of surface waters, also known as brownification, is a process of decreasing water transparency, particularly in boreal lakes surrounded by intensively managed forests and wetlands. In this paper, we review the ecological consequences and ecosystem-based management (EBM) of browning through a systematic review approach and adopt an interdisciplinary approach to formulating new governance of this complex phenomenon. To understand the effects of browning on the recreational value of freshwaters, we present primary survey data on public perceptions of recreational fishing tourists on water quality in Finland. We identify a need to develop EBM beyond the EU\'s Water Framework Directive (WFD) to fully account for the extensive implications of browning. We also highlight the need for a better understanding of the within-lake microbial processes to estimate the browning-associated changes in the greenhouse gas balance of lakes. Tourist perceptions of the quality of waterbodies in Finland were largely in agreement with the general proportion of waterbodies classified in a good or excellent ecological status class, but these perceptions may be detached from biological quality assessment criteria. Consequently, we suggest that the EBM of inland waters should improve the utilization of information on not only biogeochemical processes but also users\' perspectives on aquatic ecosystems beyond the EU WFD.

Data from monitoring programs with high spatial resolution but low temporal resolution are often overlooked when assessing temporal trends, as the data structure does not permit the use of established trend analysis methods. However, the data include uniquely detailed information about geographically differentiated temporal trends driven by large-scale influences, such as climate or airborne deposition. In this study, we used geographically weighted regression models, extended with a temporal component, to evaluate linear and nonlinear trends in environmental monitoring data. To improve the results, we tested approaches for station-wise pre-processing of data and for validation of the resulting models. To illustrate the method, we used data on changes in total organic carbon (TOC) obtained in a monitoring program of around 4800 Swedish lakes observed once every 6 years between 2008 and 2021. On applying the methods developed here, we identified nonlinear changes in TOC from consistent negative trends over most of Sweden around 2010 to positive trends during later years in parts of the country.

Reduced atmospheric acid deposition has given rise to recovery from acidification - defined as increasing pH, acid neutralization capacity (ANC), or alkalinity in surface waters. Strong evidence of recovery has been reported across North America and Europe, driving chemical responses. The primary chemical responses identified in this review were increasing concentration and changing character of natural organic matter (NOM) towards predominantly hydrophobic nature. The concentration of NOM also influenced trace metal cycling as many browning surface waters also reported increases in Fe and Al. Further, climate change and other factors (e.g., changing land use) act in concert with reductions in atmospheric deposition to contribute to widespread browning and will have a more pronounced effect as deposition stabilizes. The observed water quality trends have presented challenges for drinking water treatment (e.g., increased chemical dosing, poor filter operations, formation of disinfection by-products) and many facilities may be under designed as a result. This comprehensive review has identified key research areas to be addressed, including 1) a need for comprehensive monitoring programs (e.g., larger timescales; consistency in measurements) to assess climate change impacts on recovery responses and NOM dynamics, and 2) a better understanding of drinking water treatment vulnerabilities and the transition towards robust treatment technologies and solutions that can adapt to climate change and other drivers of changing water quality.

Dissolved organic carbon (DOC) is often related to the brownification of water in continental aquatic systems and to changes in the physiology of zooplankton organisms. Zooplankton resting eggs are particularly sensitive to changes in light and chemical characteristics of water, but the physical and chemical effects associated to DOC on dormant stages have never been tested before. Herein, we tested how DOC affects hatching rates and time to hatching of Cladocera (Diaphanosoma birgei) resting eggs. In order to analyze the chemical (i.e., toxic) and physical (i.e., light attenuation) effects of DOC on hatching patterns, resting eggs were exposed to different concentrations of DOC (0, 50, and 100 mg L-1) in an experimental design which isolated chemical from physical effects. When evaluating the physical effects of DOC, hatching was more than 150% less in 100 than 50 mg L-1 DOC and time to hatching was 66% lesser in 50 mg L-1 DOC than control. Hatchling numbers and time to hatching were not affected by DOC chemical effects. We conclude DOC effects on hatching mainly relied on light attenuation, while chemical effects were likely of minor importance. DOC may change Cladocera emergence patterns mainly through light attenuation in the water column.