■最近的研究表明,肠道菌群可能与脑肿瘤的发生有关,但缺乏直接的证据.本研究使用孟德尔随机化研究(MR)方法来探索肠道菌群与脑肿瘤之间的潜在因果关系。
■我们分析了211个肠道微生物群分类群和脑肿瘤之间的全基因组关联数据,使用现有最大的肠道菌群全基因组关联研究荟萃分析数据(n=13266),并将其与IEUOpenGWAS数据库中的脑肿瘤数据相结合。我们使用逆方差加权分析,辅以孟德尔随机化-Egger回归等方法,加权中位数估计器,简单模式,和加权模式,评估因果关系。此外,我们还进行了孟德尔随机化-Egger截距检验,Cochran的Q测试,和孟德尔随机化Steiger方向性检验,以确保分析的准确性。质量控制包括敏感性分析,水平基因多效性测试,异质性检验,和施泰格先生的方向性测试。
■我们的研究发现,特定的肠道微生物类群,如乳杆菌和梭状芽胞杆菌家族1,与脑肿瘤的发生呈正相关,而DefluviitaleaceaeUCG011属和Flavonnfractor属与脑肿瘤的发生呈负相关。孟德尔随机化-Egger截距检验表明,我们的分析不受多效性的影响(P>0.05)。
■这项研究首次揭示了肠道菌群与脑肿瘤之间的潜在因果关系,为早期脑肿瘤的防治提供了新的视角。这些发现可能有助于开发新的临床干预策略,并为未来的研究指明方向。
UNASSIGNED: Recent studies have shown that gut microbiota may be related to the occurrence of brain tumors, but direct evidence is lacking. This study used the Mendelian randomization study (MR) method to explore the potential causal link between gut microbiota and brain tumors.
UNASSIGNED: We analyzed the genome-wide association data between 211 gut microbiota taxa and brain tumors, using the largest existing gut microbiota Genome-Wide Association Studies meta-analysis data (n=13266) and combining it with brain tumor data in the IEU OpenGWAS database. We use inverse-variance weighted analysis, supplemented by methods such as Mendelian randomization-Egger regression, weighted median estimator, simple mode, and weighted mode, to assess causality. In addition, we also conducted the Mendelian randomization-Egger intercept test, Cochran\'s Q test, and Mendelian randomization Steiger directionality test to ensure the accuracy of the analysis. Quality control includes sensitivity analysis, horizontal gene pleiotropy test, heterogeneity test, and MR Steiger directionality test.
UNASSIGNED: Our study found that specific gut microbial taxa, such as order Lactobacillales and family Clostridiaceae1, were positively correlated with the occurrence of brain tumors, while genus Defluviitaleaceae UCG011 and genus Flavonifractor were negatively correlated with the occurrence of brain tumors. The Mendelian randomization-Egger intercept test showed that our analysis was not affected by pleiotropy (P>0.05).
UNASSIGNED: This study reveals for the first time the potential causal relationship between gut microbiota and brain tumors, providing a new perspective for the prevention and treatment of early brain tumors. These findings may help develop new clinical intervention strategies and point the way for future research.