背景:氘代谢成像(dourimemetalthemicimaging,damage,在施用2H标记的底物后进行的非侵入性代谢MR成像方法。[6,6'-2H2]葡萄糖消耗后的MDI已用于研究大脑代谢过程,但不同的[6,6'-2H2]葡萄糖剂量对大脑数据的影响尚不清楚。
目的:研究7T时人脑中三种不同的[6,6'-2H2]葡萄糖剂量对dmi的影响。
方法:前瞻性。
方法:6名健康参与者(年龄:28±8岁,男/女:3/3)。
■7T,3D2H自由感应衰减(FID)-磁共振波谱成像(MRSI)序列。
结果:三名受试者接受了两种不同的剂量(0.25g/kg,0.50g/kg或0.75g/kg体重)的[6,6'-2H2]葡萄糖两次,并连续进行2H-MRSI扫描120分钟。扫描期间每10分钟采集一次血样,测定血浆葡萄糖水平和血浆2H-葡萄糖原子百分比过量(APE)(部分-1)。三名受试者在接受0.50g/kg[6,6'-2H2]葡萄糖(第2部分)后接受相同的方案一次。
方法:使用单向ANOVA比较平均血浆2H-葡萄糖APE和葡萄糖血浆浓度。用两级线性混合模型分析脑2H-Glc和脑2H-Glx(部分-1)。在第2部分中,使用一般线性模型来比较脑代谢物信号。P<0.05时具有统计学意义。
结果:摄入[6,6'-2H2]葡萄糖后60至100分钟,血浆2H-GlcAPE在0.50g/kg和0.75g/kg剂量之间没有差异(P=0.961),但显著低于0.25g/kg。时间和剂量显着影响大脑2H-葡萄糖水平(对于0.25g/kg,估计值±标准误差[SE]:0.89±0.01、1.09±0.01和1.27±0.01,0.50g/kg,和0.75g/kg,分别)和大脑2H-谷氨酸/谷氨酰胺水平(对于0.25g/kg,估计值±SE:1.91±0.03、2.27±0.03和2.46±0.03,0.50g/kg,和0.75g/kg,分别)。血浆2H-GlcAPE,大脑2H-Glc,在接受0.50g/kg[6,6'-2H2]葡萄糖的受试者中,大脑2H-Glx水平相当。
结论:脑2H-葡萄糖和脑2H-谷氨酸/谷氨酰胺显示[6,6'-2H2]葡萄糖剂量依赖性。0.50g/kg的剂量证明相当,并且可以很好地检测,脑中的2H-葡萄糖和2H-谷氨酸/谷氨酰胺信号。
方法:1技术效果:阶段2。
BACKGROUND: Deuterium metabolic imaging (DMI) is an innovative, noninvasive metabolic MR imaging method conducted after administration of 2H-labeled substrates. DMI after [6,6\'-2H2]glucose consumption has been used to investigate brain metabolic processes, but the impact of different [6,6\'-2H2]glucose doses on DMI brain data is not well known.
OBJECTIVE: To investigate three different [6,6\'-2H2]glucose doses for DMI in the human brain at 7 T.
METHODS: Prospective.
METHODS: Six healthy participants (age: 28 ± 8 years, male/female: 3/3).
UNASSIGNED: 7 T, 3D 2H free-induction-decay (FID)-magnetic resonance spectroscopic imaging (MRSI) sequence.
RESULTS: Three subjects received two different doses (0.25 g/kg, 0.50 g/kg or 0.75 g/kg body weight) of [6,6\'-2H2]glucose on two occasions and underwent consecutive 2H-MRSI scans for 120 minutes. Blood was sampled every 10 minutes during the scan, to determine plasma glucose levels and plasma 2H-Glucose atom percent excess (APE) (part-1). Three subjects underwent the same protocol once after receiving 0.50 g/kg [6,6\'-2H2]glucose (part-2).
METHODS: Mean plasma 2H-Glucose APE and glucose plasma concentrations were compared using one-way ANOVA. Brain 2H-Glc and brain 2H-Glx (part-1) were analyzed with a two-level Linear Mixed Model. In part-2, a General Linear Model was used to compare brain metabolite signals. Statistical significance was set at P < 0.05.
RESULTS: Between 60 and 100 minutes after ingesting [6,6\'-2H2]glucose, plasma 2H-Glc APE did not differ between 0.50 g/kg and 0.75 g/kg doses (P = 0.961), but was significantly lower for 0.25 g/kg. Time and doses significantly affected brain 2H-Glucose levels (estimate ± standard error [SE]: 0.89 ± 0.01, 1.09 ± 0.01, and 1.27 ± 0.01, for 0.25 g/kg, 0.50 g/kg, and 0.75 g/kg, respectively) and brain 2H-Glutamate/Glutamine levels (estimate ± SE: 1.91 ± 0.03, 2.27 ± 0.03, and 2.46 ± 0.03, for 0.25 g/kg, 0.50 g/kg, and 0.75 g/kg, respectively). Plasma 2H-Glc APE, brain 2H-Glc, and brain 2H-Glx levels were comparable among subjects receiving 0.50 g/kg [6,6\'-2H2]glucose.
CONCLUSIONS: Brain 2H-Glucose and brain 2H-Glutamate/Glutamine showed to be [6,6\'-2H2]glucose dose dependent. A dose of 0.50 g/kg demonstrated comparable, and well-detectable, 2H-Glucose and 2H-Glutamate/Glutamine signals in the brain.
METHODS: 1 TECHNICAL EFFICACY: Stage 2.