■对癫痫发作发病机制的理解随着时间的推移而发展,现在人们普遍认为,不仅涉及皮质和皮质下区域,而且还涉及白质(WM)中这些区域的连接。最近的人类神经影像学研究证实了WM参与了几种癫痫综合征。缺乏通过扩散张量成像(DTI)研究犬特发性癫痫的WM完整性的神经影像学研究。这项研究旨在验证以下假设:在患有特发性癫痫的狗中可以发现WM扩散变化。
■26只特发性癫痫犬(15只边境牧羊犬和11只大瑞士山区犬)和24只健康对照(11只比格犬,5边境牧羊犬,和8只大瑞士山区犬)被前瞻性纳入。大多数特发性癫痫犬(17/26)在癫痫发作后3个月内入组。在3特斯拉扫描仪中进行具有32个扩散方向的脑扩散张量成像(低b值=0s/mm2;最大b值=800s/mm2)。基于轨道的空间统计(TBSS),基于体素的方法,与健康对照组相比,特发性癫痫组的各向异性分数(FA)和平均扩散率(MD)的变化。此外,两组均在call体和扣带白质区域进行FA和MD调查。
■我们观察到特发性癫痫组和健康对照组之间WMDTI的细微变化,仅限于扣带WM,在感兴趣区域(ROI)方法中,特发性癫痫组的FA显着低于健康对照组(p=0.027)。在TBSS分析中,特发性癫痫组与健康对照组之间以及ROI方法中的call体之间均未发现显着变化。
■这项研究支持扣带区域作为犬癫痫的目标结构。这些细微的变化只能解释为癫痫持续时间短,小样本量,以及犬脑解剖结构中更高的变异性。此外,所有纳入的狗都表现出全身性强直-阵挛性癫痫发作,可能受全身性癫痫综合征影响,与局灶性癫痫综合征相比,这也与人类不太明显的DTI变化有关。
UNASSIGNED: The understanding of epileptic seizure pathogenesis has evolved over time, and it is now generally accepted that not only are cortical and subcortical areas involved but also the connection of these regions in the white matter (WM). Recent human neuroimaging studies confirmed the involvement of the WM in several epilepsy syndromes. Neuroimaging studies investigating WM integrity with diffusion tensor imaging (DTI) in canine idiopathic epilepsy are lacking. This study aimed to test the hypothesis that WM diffusion changes can be found in dogs affected by idiopathic epilepsy.
UNASSIGNED: Twenty-six dogs with idiopathic epilepsy (15 Border Collies and 11 Greater Swiss Mountain dogs) and 24 healthy controls (11 Beagle dogs, 5 Border Collies, and 8 Greater Swiss Mountain dogs) were prospectively enrolled. Most dogs with idiopathic epilepsy (17/26) were enrolled within 3 months after seizure onset. Diffusion tensor imaging of the brain with 32 diffusion directions (low b value = 0 s/mm2; maximal b value = 800 s/mm2) was performed in a 3 Tesla scanner. Tract-based spatial statistics (TBSS), a voxel-based approach, was used to investigate changes in fractional anisotropy (FA) and mean diffusivity (MD) in the idiopathic epilepsy group compared to the healthy control group. Additionally, FA and MD were investigated in the region of corpus callosum and cingulate white matter in both groups.
UNASSIGNED: We observed subtle changes in WM DTI between the idiopathic epilepsy group and the healthy control group limited to cingulate WM, with a significantly lower FA in the idiopathic epilepsy group compared to the healthy control group in the region of interest (ROI) approach (p = 0.027). No significant changes were found between the idiopathic epilepsy group and the healthy control group in the TBSS analysis and in the corpus callosum in the ROI approach.
UNASSIGNED: This study supports the cingulate area as a target structure in canine epilepsy. The subtle changes only might be explained by the short duration of epilepsy, small sample sizes, and the higher variability in canine brain anatomy. Furthermore, all included dogs showed generalized tonic-clonic seizures, possibly affected by generalized epilepsy syndrome, which are also associated with less pronounced DTI changes in humans than focal epilepsy syndromes.