■在这项研究中,我们开发了一种运动训练方案,用于评估运动期间各种血管组织中的血压动态和嘌呤受体mRNA表达水平.目的是评估运动训练对自发性高血压大鼠(SHR)血压调节和血管组织中嘌呤受体的影响。
■将WistarKyoto(WKY)和SHR大鼠随机分为久坐(Sed)和运动训练(ExT)组。Sed组的大鼠被允许不受限制的运动,而ExT组的患者接受了16周的低至中等强度跑步机运动方案。在整个干预期间,进行血压测量和体重记录.此外,嘌呤受体P2X1、P2Y1、P2Y2mRNA在肾动脉中的表达,颈内动脉(Int),胸主动脉(Aor),评估尾动脉(Cau)组织。
■在Sed组中,观察到SHR大鼠的体重低于其他三组。在锻炼的过程中,SHR大鼠ExT组血压逐渐降低,在运动期结束时,收敛到与WKY大鼠相似的水平。关于P2X1受体在四个血管中的mRNA表达模式,WKY和SHR大鼠表现出相似的序列,始终在Cau中显示最高表达水平。相反,在WKY和SHR大鼠中,P2Y1和P2Y2受体的mRNA表达在四个血管中显示出不同的序列。值得注意的是,与Sed组的WKY大鼠相比,SHR大鼠Int中P2X1受体的mRNA表达增加,运动后,WKY大鼠Aor和SHR大鼠Cau的表达下降。在SHR大鼠的所有四种类型的血管中,P2Y1受体mRNA的表达均降低。锻炼后,P2Y1受体mRNA在Aor中表达增加,在WKY大鼠的Cau中降低,并在SHR大鼠的Int和肾动脉(RA)中增加。相反,SHR大鼠Int和Aor中P2Y2受体mRNA的表达降低。除了WKY大鼠的Aor,运动后,两种大鼠其他动脉中P2Y2受体mRNA的表达均增加。
■观察到WKY和SHR大鼠不同动脉段中嘌呤受体亚型分布的差异。发现运动训练可增强这些大鼠模型中P2Y受体的mRNA表达水平。这一发现表明,运动训练可能通过增强嘌呤能放松反应来减轻SHR大鼠的高血压。
UNASSIGNED: In this study, we developed an exercise training protocol for assessing both blood pressure dynamics and mRNA expression levels of purine receptors in various vascular tissues during physical activity. The objective is to assess the impact of exercise training on blood pressure regulation in spontaneously hypertensive rats (SHR) and purine receptors in vascular tissues.
UNASSIGNED: Wistar Kyoto (WKY) and SHR rats were randomly allocated into sedentary (Sed) and exercise training (ExT) groups. Rats in the Sed groups were allowed unrestricted movement, whereas those in the ExT groups underwent a 16-week regimen of low- to moderate-intensity treadmill exercise. Throughout the intervention period, blood pressure measurements and body weight recordings were conducted. Additionally, mRNA expressions of purine receptors P2X1, P2Y1, and P2Y2 in renal artery (RA), internal carotid artery (Int), thoracic aorta (Aor), and caudal artery (Cau) tissues were assessed.
UNASSIGNED: In the Sed group, body weight of SHR rats was observed to be lower compared to the three other groups. Over the course of the exercise regimen, blood pressure in the ExT group of SHR rats reduced gradually, converging towards levels similar to those observed in WKY rats by the conclusion of the exercise period. Regarding mRNA expression patterns of P2X1 receptors across the four blood vessels, WKY and SHR rats demonstrated similar sequences, consistently displaying the highest expression levels in the Cau. Conversely, mRNA expressions of P2Y1 and P2Y2 receptors exhibited distinct sequences across the four blood vessels in both WKY and SHR rats. Notably, compared to the Sed group of WKY rats, mRNA expression of P2X1 receptor in the Int of SHR rats revealed an increase, while expressions in the Aor of WKY rats and the Cau of SHR rats decreased following exercise. Expression of P2Y1 receptor mRNA decreased across all four types of blood vessels in SHR rats. Post-exercise, P2Y1 receptor mRNA expression increased in the Aor, decreased in the Cau of WKY rats, and increased in the Int and renal artery (RA) of SHR rats. Conversely, expressions of P2Y2 receptor mRNA decreased in the Int and Aor of SHR rats. Except for the Aor of WKY rats, expressions of P2Y2 receptor mRNA increased in the other arteries of both rat types following exercise.
UNASSIGNED: Differences in the distribution of purine receptor subtypes among distinct arterial segments in both WKY and SHR rats were observed. Exercise training was found to enhance mRNA expression levels of P2Y receptors in these rat models. This finding implies that exercise training might reduce hypertension in SHR rats by bolstering the purinergic relaxation response.