背景:由于各种优点,在不同的工业应用中,酶催化通常比化学方法更受欢迎,如更高的特异性,更高的效率,更少的环境足迹。果胶酶是一组催化果胶化合物降解的酶,植物中层板和初生细胞壁的关键成分。果胶酶已在多种工业过程中找到应用,包括棉花生物精练,果汁的提取及其澄清,植物纤维脱胶,造纸,植物生物质液化,和糖化,在其他人中。这项研究的目的是对显示果胶分解活性的细菌物种进行分类学表征,并定性和定量地评估其果胶分解活性,以及测试其生物精练潜力。
结果:这里,我们报道了洋葱伯克霍尔德菌,一种以前未知的具有果胶分解活性的物种,发挥与纺织工业中商业使用的果胶酶相当的活性,但需要较少的活动温度。
结论:酶活性的定量评估表明细菌物种用于棉针织物的生物精练的潜力。
BACKGROUND: Enzymatic catalysis in different industrial applications is often preferred over chemical methods due to various advantages, such as higher specificity, greater efficiency, and less environmental footprint. Pectinases are a group of enzymes that catalyze the degradation of pectic compounds, the key components of plant middle lamella and the primary cell wall. Pectinases have found applications in multiple industrial processes, including cotton
bioscouring, fruit juice extraction and its clarification, plant fiber degumming, paper making, plant biomass liquefaction, and saccharification, among others. The purpose of this study was to taxonomically characterize a bacterial species exhibiting pectinolytic activities and assess its pectinolytic activity qualitatively and quantitatively, as well as test its
bioscouring potential.
RESULTS: Here, we report that Burkholderia cepacia, a previously unknown species with pectinolytic activity, exerts such activity comparable to commercially used pectinase enzymes in the textile industry, but requires less temperature for activity.
CONCLUSIONS: Quantitative evaluation of enzyme activity indicates the potential of the bacterial species for use in the
bioscouring of cotton knit fabric.