硒纳米球(SeNPs)显示比亚硒酸盐更低的毒性和更高的生物利用度。这项研究证明了植物乳杆菌NML21对Se(IV)的实质性耐受性和有效转化为SeNPs。Se(IV)的生物还原过程和SeNPs的性质,包括它们的形态,颗粒大小,和稳定性,用包括SEM在内的技术进行了研究,EDX,TEM,XPS,FT-IR,动态光散射,XRD,和拉曼光谱。在高硒胁迫下,某些细胞表现出明显的变形和破裂,并释放出SeNPs作为Se(IV)生物还原的主要产物。这些SeNPs是红色的,无定形,零价,和球形,平均直径为160nm。光谱分析强调CO和CO的官能团是Se(IV)生物还原的关键。该研究提出了乳酸菌生物还原Se(IV)以及SeNPs形成和释放的初步机制。因此,NML21可能是SeNPs合成的有希望的候选物。
Selenium nanospheres (SeNPs) show less toxicity and greater bioavailability than selenite salts. This research demonstrated the substantial tolerance and efficient conversion of Se(IV) into SeNPs by Lactiplantibacillus plantarum NML21. The
bioreduction process of Se(IV) and the properties of SeNPs, including their morphology, particle size, and stability, were investigated with techniques including SEM, EDX, TEM, XPS, FT-IR, dynamic light scattering, XRD, and Raman spectroscopy. Under high selenium stress, certain cells displayed significant deformation and rupture, and released SeNPs as the main product of the
bioreduction of Se(IV). These SeNPs were red, amorphous, zero-valent, and spherical, with an average diameter of 160 nm. Spectroscopic analysis highlighted that the functional groups of CO and CO are key to the
bioreduction of Se(IV). The study suggested preliminary mechanisms for the
bioreduction of Se(IV) and the formation and release of SeNPs by lactic acid bacteria. NML21 may therefore be a promising candidate for SeNPs synthesis.