细胞培养在大规模的高附加值生物药物的工业生产中经常是优选的。在生物制药的生产中,选择合适的细胞系,细胞培养基,文化条件非常重要。在供人类使用的医疗产品中,认可机构不允许使用增加培养产量的血清,添加到文化中。出于这个原因,所使用的细胞系必须适应无血清培养基。在这项研究中,首先,THP-1细胞系的直接和逐渐适应,它在生物制药生产中具有很高的潜力,对当地生产的化学定义的无血清培养基进行。然后,比较摇瓶与市售两种不同的无血清培养基的生产效率。最后,第一次,在旋转烧瓶和搅拌罐生物反应器中产生THP-1细胞,以模拟这项工作范围内的大规模。
Cell cultures are frequently preferred in the industrial production of high value-added biopharmaceuticals on a large scale. In the production of biopharmaceuticals, the selection of the appropriate cell line, the cell culture medium, and the culture conditions are very important. In medical products to be offered for human use, authorized institutions do not allow the use of serum which increases the culture yield, added to the culture. For this reason, the cell lines to be used must be adapted to the serum-free medium. In this study, first, the direct and gradual adaptation of the THP-1 cell line, which has a high potential for use in biopharmaceutical production, to a locally produced chemically defined serum-free medium was carried out. Then, a comparison of the production efficiency in the shake flasks with the commercially available two different serum-free media was performed. Finally, for the first time, THP-1 cells were produced in spinner flasks and stirring tank bioreactor to simulate the large scale within the scope of this work.