未经证实:肺鳞状细胞癌(LUSC)是肺部恶性肿瘤的主要亚型,与全球癌症介导的死亡率有关。然而,与肿瘤的生存预后和免疫力相关的转录组特征的鉴定仍然缺乏。
未经鉴定:整合了GSE2088,GSE6044,GSE19188,GSE21933,GSE33479,GSE33532和GSE74706,以鉴定具有组合效应大小的差异表达基因(DEGs)。此外,TCGALUSC队列用于进一步分析.利用一系列生物信息学方法进行整个研究。
UNASSIGNED:发现831个基因(如DSG3,PKP1,DSC3,TPX2和UBE2C)上调,731个基因(如ABCA8,SELENBP1,FAM107A,和CACNA2D2)在LUSC中下调。功能富集分析确定了上调的KEGG途径,包括细胞周期,DNA复制,基底切除修复,蛋白酶体,失配修复,和细胞衰老。此外,关键枢纽基因(如EGFR,HRAS,JUN,CDH1,BRCA1,CAP3,RHOA,HDAC1,HIF1A,和CCNA2)以及与蛋白质-蛋白质相互作用(PPI)显着相关的八个基因模块。临床分析发现,CDH3、PLAU的过表达组,PKP3,STIL,CALU,LOXL2,POSTN,DPP3,GALNT2,LOX,和ITPA与不良生存预后显著相关,IL18R1下调组显示出相似的趋势.此外,我们的研究表明,生存相关基因与LUSC的基质和免疫评分相关,表明生存相关基因调节肿瘤免疫。27%的LUSC患者的生存相关基因发生了遗传改变,并显示出出色的诊断效率。最后,CDH3,PLAU的一致表达水平,PKP3,STIL,CALU,LOXL2,POSTN,在TCGALUSC队列中发现了DPP3、GALNT2和ITPA。
UNASSIGNED:关键转录组特征的鉴定可以通过LUSC癌变的关键机制来阐明。
UNASSIGNED: Lung Squamous Cell Carcinoma (LUSC) is a major subtype of lung malignancies and is associated with the cause of cancer-mediated mortality worldwide. However, identification of transcriptomic signatures associated with survival-prognosis and immunity of tumor remains lacking.
UNASSIGNED: The GSE2088, GSE6044, GSE19188, GSE21933, GSE33479, GSE33532, and GSE74706 were integrated for identifying differentially expressed genes (DEGs) with combined effect sizes. Also, the TCGA LUSC cohort was used for further analysis. A series of bioinformatics methods were utilized for conducting the whole study.
UNASSIGNED: The 831 genes (such as DSG3, PKP1, DSC3, TPX2, and UBE2C) were found upregulated and the 731 genes (such as ABCA8, SELENBP1, FAM107A, and CACNA2D2) were downregulated in the LUSC. The functional enrichment analysis identifies the upregulated KEGG pathways, including cell cycle, DNA replication, base excision repair, proteasome, mismatch repair, and cellular senescence. Also, the key hub genes (such as EGFR, HRAS, JUN, CDH1, BRCA1, CASP3, RHOA, HDAC1, HIF1A, and CCNA2) were identified along with the eight gene modules that were significantly related to the protein-protein interaction (PPI). The clinical analyses identified that the overexpression group of CDH3, PLAU, PKP3, STIL, CALU, LOXL2, POSTN, DPP3, GALNT2, LOX, and ITPA are substantially associated with a poor survival prognosis and the downregulated group of IL18R1 showed a similar trend. Moreover, our investigation demonstrated that the survival-associated genes were correlated with the stromal and immune scores in LUSC, indicating that the survival-associated genes regulate tumor immunity. The survival-associated genes were genetically altered in 27% of LUSC patients and showed excellent diagnostic efficiency. Finally, the consistent expression level of CDH3, PLAU, PKP3, STIL, CALU, LOXL2, POSTN, DPP3, GALNT2, and ITPA were found in the TCGA LUSC cohort.
UNASSIGNED: The identification of key transcriptomic signatures can be elucidated by the crucial mechanism of LUSC carcinogenesis.