在这项研究中,使用来自子囊门的真菌物种获得了七个不同的银纳米颗粒(AgNPs),管曲霉,曲霉属。,枝状孢子菌,镰刀菌增殖,Epicoccumnigrum,Exserohilumrostratum,和OchroleucaBionectria,与巴西的生物多样性隔绝,特别是来自红树林和卡廷加生物群落。纳米粒子被编码为AgNP-AT,AgNP-Asp,AgNP-CPP,AgNP-FP,AgNP-EN,AgNP-ER,和AgNP-BO,并使用分光光度法(UV-Vis)进行表征,动态光散射(DLS),zeta电位,透射电子显微镜(TEM),和傅里叶变换红外(FTIR)光谱。所有的AgNPs呈现均匀的尺寸范围从43.4到120.6nm(DLS)和从21.8到35.8nm(TEM),pH从4.5到7.5,负电荷,并且在它们的表面上存在蛋白质涂层。在念珠菌的临床菌株上评估了AgNP的抗真菌活性,关于非白色念珠菌物种,克鲁斯念珠菌,光滑念珠菌,近带念珠菌,热带念珠菌,还有念珠菌,常见于医院感染,对抗植物病原体尖孢镰刀菌,镰刀菌,镰刀菌,镰刀菌谷蛋白,轮状镰刀菌,和弯孢菌,是对农业生产造成严重破坏的物种。AgNPs对MIC范围为1.25至40µM的酵母和MIC范围为4至250µM的植物病原体有效,表明这些AgNPs作为抗真菌剂的应用有希望的可能性。结果表明,AgNPs的理化参数,包括其表面上存在的官能团,干扰了它们的抗真菌活性。总的来说,结果表明,AgNPs对酵母或植物病原体没有特异性,这可能是一个优势,增加在不同领域应用的可能性。
In this study, seven different silver nanoparticles (AgNPs) were obtained using the fungi species from the phylum Ascomycota, Aspergillus tubingensis, Aspergillus spp., Cladosporium pini-ponderosae, Fusarium proliferatum, Epicoccum nigrum, Exserohilum rostratum, and Bionectria ochroleuca, isolated from the Brazilian biodiversity, particularly from the mangrove and Caatinga biomes. The nanoparticles were coded as AgNP-AT, AgNP-Asp, AgNP-CPP, AgNP-FP, AgNP-EN, AgNP-ER, and AgNP-BO and characterized using spectrophotometry (UV-Vis), dynamic light scattering (DLS), zeta potential, transmission electron microcopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy. All the AgNPs presented homogeneous size in the range from 43.4 to 120.6 nm (DLS) and from 21.8 to 35.8 nm (TEM), pH from 4.5 to 7.5, negative charge, and presence of protein coating on their surface. The antifungal activity of the AgNPs was evaluated on clinical strains of Candida albicans, and on the non-albicans species, Candida krusei, Candida glabrata, Candida parapsilosis, Candida tropicalis, and Candida guilliermondii, common in hospital infections, and against the phytopathogens Fusarium oxysporum, Fusarium phaseoli, Fusarium sacchari, Fusarium subglutinans, Fusarium verticillioides, and Curvularia lunata, which are species responsible for serious damage to agriculture production. The AgNPs were effective against the yeasts with MICs ranging from 1.25 to 40 µM and on the phytopathogens with MICs from 4 to 250 µM, indicating the promising possibility of application of these AgNPs as antifungal agents. The results indicated that the physicochemical parameters of the AgNPs, including the functional groups present on their surface, interfered with their antifungal activity. Overall, the results indicate that there is no specificity of the AgNPs for the yeasts or for the phytopathogens, which can be an advantage, increasing the possibility of application in different areas.