目的:黑皮病,由solani根瘤菌引起,是一种严重的土壤传播和块茎传播疾病,在全球马铃薯种植区发生和传播,对马铃薯生产构成严重威胁。新的生物杀菌剂是非常需要解决的问题,和来自Xenorhabdusspp的天然产物(NPs)。为生物杀菌剂的开发提供丰富的资源。在这项研究中,我们的目的是从Xenorhabdusspp中鉴定抗真菌NPs。这种疾病的管理。
结果:在所调查的22种Xenorhabdus菌株中,Xenorhabdusbudapestenensis8(XBD8)被确定为最有希望的候选物,其无细胞上清液(CFS)对枯草杆菌的IC50值低至0.19mLL-1。XBD8中的主要抗真菌化合物在对数中期开始合成,并在稳定期达到稳定水平。核心基因缺失与高分辨率质谱(HRMS/MS)分析相结合,确定了主要的抗真菌NPs为fabclavine衍生物,Fcl-7和8,对重要病原真菌显示出广谱生物活性。令人印象深刻的是,在温室和田间试验中,鉴定出的法克林衍生物有效地控制了黑毛皮病,与化学杀菌剂氟二恶英相当,可显着改善块茎质量,并以可销售的块茎产量从29.300kgha-1增加到35.494kgha-1。
结论:已确定Fabclavine衍生物Fcl-7和Fcl-8是XBD8中主要的抗真菌NPs,这显示了治疗黑皮病的光明前景。
OBJECTIVE: Black scurf disease, caused by Rhizoctonia solani, is a severe soil-borne and tuber-borne disease, which occurs and spreads in potato growing areas worldwide and poses a serious threat to potato production. New
biofungicide is highly desirable for addressing the issue, and natural products (NPs) from Xenorhabdus spp. provide prolific resources for
biofungicide development. In this study, we aim to identify antifungal NPs from Xenorhabdus spp. for the management of this disease.
RESULTS: Out of the 22 Xenorhabdus strains investigated, Xenorhabdus budapestensis 8 (XBD8) was determined to be the most promising candidate with the measured IC50 value of its cell-free supernatant against R. solani as low as 0.19 ml l-1. The major antifungal compound in XBD8 started to be synthesized in the middle logarithmic phase and reached a stable level at stationary phase. Core gene deletion coupled with high-resolution mass spectrometry analysis determined the major antifungal NPs as fabclavine derivatives, Fcl-7 and 8, which showed broad-spectrum bioactivity against important pathogenic fungi. Impressively, the identified fabclavine derivatives effectively controlled black scurf disease in both greenhouse and field experiments, significantly improving tuber quality and increasing with marketable tuber yield from 29 300 to 35 494 kg ha-1, comparable with chemical fungicide fludioxonil.
CONCLUSIONS: The fabclavine derivatives Fcl-7 and 8 were determined as the major antifungal NPs in XBD8, which demonstrated a bright prospect for the management of black scurf disease.