马铃薯是一种重要的作物,由于其淀粉含量高,蛋白质,以及各种维生素和矿物质。生物肥料由植物生长促进微生物(PGPM)组成,这些微生物对于提高马铃薯的生长和抗性至关重要。然而,很少有信息关注生物肥料对植物生长和微生态的接种方式。本研究旨在揭示马铃薯对三种含PGPM解淀粉芽孢杆菌EZ99的生物肥料接种模式的响应机制,即5kg/ha生物肥料(M5)的分散模式,用溶解的5kg/ha生物肥料(MZG)浸泡种子块茎,3kg/ha生物肥+2kg/ha蔗糖(MY34)在碱性黄土田的分散模式,代谢组和微生物组。生理结果表明,M5和MZG两种等量施用方式显著提高了马铃薯的生长和产量。此外,马铃薯的转录组表现出富含光合作用的差异表达基因集,糖代谢,和苯丙素生物合成的三种模式,M5模式表现出828个基因的整体上调。基于马铃薯块茎的非靶向代谢组学分析,M5模式显著积累蔗糖,而MZG和MY34模式显著积累了应激代谢产物echrenoneb6和甘露二糖,分别。此外,马铃薯根际微生物结构表明,所有土壤中细菌和真菌的多样性相似,但是它们的丰度差异很大。具体来说,有益青霉在M5和MZG土壤中富集,而MY34土壤中积累了潜在的病原菌斑孢菌和嗜糖被孢霉。总的来说,这些发现强调了MZG是促进马铃薯生长和刺激根际效应的最有效模式。本研究不仅通过农业生态实践鼓励可持续农业,同时也为PGPM生物肥料在主食中的应用提供了广阔的前景。
Potato is an important crop due to its high contents of starch, protein, and various vitamins and minerals. Biofertilizers are composed of plant growth promoting microbes (PGPMs) which are essential for improving the growth and resistance of potato. However, little information has focused on the modes of inoculation of biofertilizers on plant growth and microecology. This study aims to reveal the response mechanism of the potato to three modes of inoculation of biofertilizers all containing PGPM Bacillus amyloliquefaciens EZ99, i.e. scattered mode of 5 kg/ha
biofertilizer (M5), soaking seed tubers with dissolved 5 kg/ha
biofertilizer (MZG), and scattered mode of 3 kg/ha
biofertilizer + 2 kg/ha sucrose (MY34) in alkaline loess field through multi-omics analysis of transcriptome, metabolome and microbiome. The physiological result revealed that two application modes of equal amount of
biofertilizer M5 and MZG significantly improved the growth and yield of potatoes. Furthermore, the transcriptome of potato exhibited sets of differentially expressed genes enriched in photosynthesis, sugar metabolism, and phenylpropanoid biosynthesis among the three modes, with the M5 mode exhibiting overall up-regulation of 828 genes. Based on the untargeted metabolomic analysis of potato tuber, M5 mode significantly accumulated sucrose, while MZG and MY34 mode significantly accumulated the stress metabolites euchrenone b6 and mannobiose, respectively. Besides, the microbial structure of potato rhizosphere showed that the diversity of bacteria and fungi was similar in all soils, but their abundances varied significantly. Specifically, beneficial Penicillium was enriched in M5 and MZG soils, whereas MY34 soil accumulated potential pathogens Plectosphaerella and saccharophilic Mortierella. Collectively, these e findings highlight that MZG is the most effective mode to promote potato growth and stimulate rhizosphere effect. The present study not only encourages sustainable agriculture through agroecological practices, but also provides broad prospects for the application of PGPM
biofertilizer in staple foods.