Bio-sensors

生物传感器
  • 文章类型: Journal Article
    珊瑚礁是各种物种的家园,它们的保存是一个受欢迎的研究领域;然而,监控它们是一个巨大的挑战,机器人的使用提供了一个有希望的答案。这项研究的目的是分析目前在珊瑚礁监测中使用的技术和工具,重点关注机器人技术的作用及其在改变这一领域中的潜力。使用系统的审查方法,检查了Scopus数据库中有关“机器人”和“珊瑚礁”关键字的工程和地球科学的同行评审文献,文章分为三个部分:珊瑚礁监测,机器人在珊瑚礁监测中,和案例研究。初步调查结果表明了多种监测策略,每个人都有自己的优点和缺点。案例研究还强调了机器人技术在监测中的全球应用,强调每个背景下独特的挑战和机遇。人工智能和机器学习驱动的机器人干预导致了珊瑚礁监测的新时代。这种事态发展不仅改善了监测,而且支持了这些脆弱生态系统的保护和恢复。需要进一步的研究,特别是在室内和公海环境中监测珊瑚苗圃和最大限度地提高珊瑚健康的机器人系统上。
    Coral reefs are home to a variety of species, and their preservation is a popular study area; however, monitoring them is a significant challenge, for which the use of robots offers a promising answer. The purpose of this study is to analyze the current techniques and tools employed in coral reef monitoring, with a focus on the role of robotics and its potential in transforming this sector. Using a systematic review methodology examining peer-reviewed literature across engineering and earth sciences from the Scopus database focusing on \"robotics\" and \"coral reef\" keywords, the article is divided into three sections: coral reef monitoring, robots in coral reef monitoring, and case studies. The initial findings indicated a variety of monitoring strategies, each with its own advantages and disadvantages. Case studies have also highlighted the global application of robotics in monitoring, emphasizing the challenges and opportunities unique to each context. Robotic interventions driven by artificial intelligence and machine learning have led to a new era in coral reef monitoring. Such developments not only improve monitoring but also support the conservation and restoration of these vulnerable ecosystems. Further research is required, particularly on robotic systems for monitoring coral nurseries and maximizing coral health in both indoor and open-sea settings.
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  • 文章类型: Journal Article
    调用密度泛函理论(DFT)来研究规范(CN)和非规范(NC)碱基与原始Si2BN(Si2BN)和磷掺杂的Si2BN(P-dop-Si2BN)之间的相互作用。通过吸附能计算确定更好的传感底物,这表明磷原子的掺杂提高了AT(-83.74kcal/mol)AU(-82.77kcal/mol)和GC(-96.36kcal/mol)碱基对的吸附强度。CN和NC基底具有比先前报道的值更高的吸附能,这得出结论P-dop-Si2BN片将是感测基底的最佳基底。同时,选定的CN和NC(次黄嘌呤除外)碱基以平行方式与薄片相互作用,这推断了与Si2BN和P-dop-Si2BN薄片的π-π相互作用。P-dop-Si2BN配合物的能隙变化(ΔEg%)有明显的变化,范围从-24.75到-197.28%,这将P-dop-Si2BN片的灵敏度提高到CN和NC碱基的检测。自然群体分析(NPA)和电子密度差图(EDDM)证实电荷从CN和NC碱基转移到Si2BN和P-dop-Si2BN片材。P-dop-Si2BN配合物的光学性质表明,在可见和近红外区域(778nm至1143nm)中观察到明显的红移和蓝移。因此,以上结果得出结论,P-dop-Si2BN片层是检测CN和NC碱基的潜在候选者,其有助于生物传感器和DNA/RNA测序装置的开发。由RamaswamyH.Sarma沟通。
    Density functional theory (DFT) is invoked to investigate the interaction between the canonical (CN) and non-canonical (NC) bases with pristine Si2BN (Si2BN) and Phosphorous-doped Si2BN (P-dop-Si2BN) sheets. Inquest for the better sensing substrate is decided through the adsorption energy calculation which reveals that doping of phosphorous atom enhances the adsorption strength of AT (-83.74 kcal/mol) AU (-82.77 kcal/mol) and GC (-96.36 kcal/mol) base pairs. The CN and NC bases have higher adsorption energy than the previous reported values which concludes that the P-dop-Si2BN sheet will be optimal substrate to sense the bases. Meanwhile, the selected CN and NC (except hypoxanthine) bases interact with sheet in parallel manner which infers the π-π interaction with Si2BN and P-dop-Si2BN sheets. The energy gap variation (ΔEg%) of the P-dop-Si2BN complexes has a noticeable change, ranging from -24.75 to -197.28% which thrust the sensitivity of the P-dop-Si2BN sheet over the detection of CN and NC bases. The natural population analysis (NPA) and electron density difference map (EDDM) confirms that charges are transferred from CN and NC bases to Si2BN and P-dop-Si2BN sheet. The optical property of the P-dop-Si2BN complexes reveals that the noticeable red and blue shift in the visible and near-infrared regions (778 nm to 1143 nm) has been observed. Therefore, the above results conclude that the P-dop-Si2BN sheet plays a potential candidate to detect the CN and NC bases which contribute to the development of biosensors and DNA/RNA sequencing devices.Communicated by Ramaswamy H. Sarma.
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  • 文章类型: Journal Article
    法诺共鸣,以其独特的非对称线形而闻名,在光子学中获得了极大的关注,特别是在传感应用。然而,用简单的几何结构来实现Fano参数的可控仍然是困难的。这里,提出并实验证明了一种使用具有多孔层的薄膜光学Fano谐振器生成从准洛伦兹到洛伦兹再到Fano的整个光谱形状的新方法。掠射角沉积技术用于创建偏振相关的Fano谐振器。通过改变s-和p-极化之间的线性极化,演示了在准洛伦兹状态和负Fano状态之间可切换的Fano设备。光谱形状的这种变化对于检测具有低折射率的材料是有利的。进行了生物颗粒感测实验,该实验证明了增强的信噪比和预测精度。最后,由于众多参数之间复杂的相互作用,优化基于薄膜的Fano谐振器的挑战,包括层厚度,孔隙度,和材料选择,已解决。逆设计工具是基于多层感知器模型开发的,该模型可以快速计算所有范围的Fano参数。与传统的穷举枚举方法(MVF=0.37)相比,该方法提高了平均验证因子(MVF=0.07,q-q')的准确性。
    Fano resonance, known for its unique asymmetric line shape, has gained significant attention in photonics, particularly in sensing applications. However, it remains difficult to achieve controllable Fano parameters with a simple geometric structure. Here, a novel approach of using a thin-film optical Fano resonator with a porous layer to generate entire spectral shapes from quasi-Lorentzian to Lorentzian to Fano is proposed and experimentally demonstrated. The glancing angle deposition technique is utilized to create a polarization-dependent Fano resonator. By altering the linear polarization between s- and p-polarization, a switchable Fano device between quasi-Lorentz state and negative Fano state is demonstrated. This change in spectral shape is advantageous for detecting materials with a low-refractive index. A bio-particle sensing experiment is conducted that demonstrates an enhanced signal-to-noise ratio and prediction accuracy. Finally, the challenge of optimizing the film-based Fano resonator due to intricate interplay among numerous parameters, including layer thicknesses, porosity, and materials selection, is addressed. The inverse design tool is developed based on a multilayer perceptron model that allows fast computation for all ranges of Fano parameters. The method provides improved accuracy of the mean validation factor (MVF = 0.07, q-q\') compared to the conventional exhaustive enumeration method (MVF = 0.37).
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  • 文章类型: Journal Article
    Silicene,石墨烯的硅对应物,哈斯被预测拥有无质量的狄拉克费米子。 与石墨烯相比,与石墨烯 相比,有效的自旋-轨道相互作用是相当显著的,因此,硅烯中的屈曲在狄拉克点打开1.55meV的间隙。该带隙可以通过施加平面应力来进一步定制,外部电场,化学官能化和 缺陷。这一特殊特性使硅烯及其各种衍生物成为 器件应用的潜在候选者。在这篇简短的评论中,我们想探索原始 硅烯及其可能的纳米衍生物的运输特征。此外,将重点介绍
硅烯及其异质结构的生物传感器应用的最新进展。
我们希望从最近的
硅烯的实验和理论研究中获得的结果将在各种应用中建立基准,例如
在自旋电子学中,生物传感和光电设备。
    Silicene, a silicon counterpart of graphene, has been predicted to possess Dirac fermions. The effective spin-orbit interaction in silicene is quite significant compared to graphene; as a result, buckled silicene exhibits a finite band gap of a few meV at the Dirac point. This band gap can be further tailored by applying in plane strain, an external electric field, chemical functionalization and defects. This special feature allows silicene and its various derivatives as potential candidates for device applications. In this topical review, we would like to explore the transport features of the pristine silicene and its possible nano derivatives. As a part of it, Thermoelectric properties as well as several routes for thermoelectric enhancement in silicene are investigated. Besides, the recent progress in biosensing applications of silicene and its hetero-structures will be highlighted. We hope the results obtained from recent experimental and theoretical studies in silicene will setup a benchmark in diverse applications such as in spintronics, bio-sensing and opto-electronic devices.
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  • 文章类型: Journal Article
    生物体已经开发出有效的机制来感知外部环境。人类设计的生物传感器利用这种自然优化,其中不同的生物机器已被调整以检测用户定义的分子的存在。具体来说,模型生物酿酒酵母中的信息素途径代表了作为合成信号系统的合适候选者。的确,它只表达一个G蛋白偶联受体(GPCR),Ste2,能够识别信息素并启动信息素依赖性基因的表达。迄今为止,设计该系统的标准程序依赖于酵母GPCR与另一个GPCR的替代以及酵母G蛋白的修饰以结合插入的受体。这里,我们提出了一种创新的计算程序,基于蛋白质结合袋的几何和化学优化,选择使天然酵母GPCR能够识别用户定义的配体所需的氨基酸取代。这个程序将允许酵母识别广泛的配体,没有关于识别它们或相应G蛋白的GPCR的先验知识。我们使用蒙特卡罗模拟在Ste2上设计了一个能够识别肾上腺素的结合袋,被选为测试配体。我们通过分子对接和分子动力学验证了Ste2突变体。我们验证了我们鉴定的氨基酸取代使Ste2能够适应并保持与肾上腺素的牢固结合。我们的结果表明,我们有效地采样了可能的突变体的巨大空间,提出了这样一种策略,作为开发新型酿酒酵母生物传感器的一个有希望的起点。
    Organisms have developed effective mechanisms to sense the external environment. Human-designed biosensors exploit this natural optimization, where different biological machinery have been adapted to detect the presence of user-defined molecules. Specifically, the pheromone pathway in the model organism Saccharomyces cerevisiae represents a suitable candidate as a synthetic signaling system. Indeed, it expresses just one G-Protein Coupled Receptor (GPCR), Ste2, able to recognize pheromone and initiate the expression of pheromone-dependent genes. To date, the standard procedure to engineer this system relies on the substitution of the yeast GPCR with another one and on the modification of the yeast G-protein to bind the inserted receptor. Here, we propose an innovative computational procedure, based on geometrical and chemical optimization of protein binding pockets, to select the amino acid substitutions required to make the native yeast GPCR able to recognize a user-defined ligand. This procedure would allow the yeast to recognize a wide range of ligands, without a-priori knowledge about a GPCR recognizing them or the corresponding G protein. We used Monte Carlo simulations to design on Ste2 a binding pocket able to recognize epinephrine, selected as a test ligand. We validated Ste2 mutants via molecular docking and molecular dynamics. We verified that the amino acid substitutions we identified make Ste2 able to accommodate and remain firmly bound to epinephrine. Our results indicate that we sampled efficiently the huge space of possible mutants, proposing such a strategy as a promising starting point for the development of a new kind of S.cerevisiae-based biosensors.
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  • 文章类型: Journal Article
    Ferrite magnetic nanoparticles (FMNPs) are gaining popularity to design biosensors for high-performance clinical diagnosis. The fusion of information shows that FMNPs based biosensors require well-tuned FMNPs as detection probes to produce large and specific biological signals with minimal non-specific binding. Nevertheless, there is a noticeable lacuna of information to solve the issues related to suitable synthesis route, particle size reduction, functionalization, sensitivity towards targeted intercellular biological tiny particles, and lower signal-to-noise ratio. Therefore it allows exploring unique characteristics of FMNPs to design a suitable sensing device for intracellular measurements and diseases detection. This review focuses on the extensively used synthesis routes, their advantages and limitations, crystalline structure, functionalization, along with recent applications of FMNPs in biosensors, taking into consideration their analytical figures of merit and range of linearity. This work also addresses the current progress, key factors for sensitivity, selectivity and productivity improvement along with the challenges, future trends and perspectives of FMNPs based biosensors.
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  • 文章类型: Journal Article
    本文对石墨烯场效应晶体管(G-FET)的最新进展进行了全面的综述,考虑到制造等各个方面,建模和仿真工具和应用,特别是在传感器中,突出未来的方向。遵守摩尔定律,为了增加集成电路的晶体管密度,已经尝试了新的替代制造材料,而不是硅由于其限制在减少晶体管的尺寸。石墨烯,一种这样的材料,由于优越的载流子迁移率和非常高的跨导增益等因素,被证明是硅的合适替代品,等,G-FET正在成为高速模拟VLSI的最合适选择,射频,和生物传感器电路。
    This paper presents a comprehensive survey on the recent developments in Graphene Field Effect Transistor (G-FET), considering various aspects such as fabrication, modelling and simulation tools and applications especially in sensors, highlighting the future directions. Complying with the Moore\'s law, to increase the transistor density of an Integrated Circuit, new alternate materials for fabrication have been tried, instead of silicon due to its limitations in reducing transistor dimensions. Graphene, one such material, proves to be a suitable alternate for silicon due to the factors like superior carrier mobility and very high trans-conductance gain, etc and G-FET is becoming the most suitable choice for high-speed analog VLSI, RF, and bio- sensor circuits.
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  • 文章类型: Journal Article
    在高浓度下,离子物质在体内可能是有毒的,催化不需要的生物反应,抑制酶,产生自由基,除了与阿尔茨海默氏症和癌症等疾病有关。尽管痕量的离子物种在环境中无处不在,在工业和农业废物径流中经常发现高浓度的这些金属。因此,开发能够快速准确检测痕量离子物质的技术仍然是全球的兴趣,特别是在天然含有其他竞争/抑制离子的水性环境中。在这里,我们概述了已经开发的技术,包括一般理论,设计,以及与离子选择性电极技术相关的好处/挑战(载体掺杂膜,碳基品种,酶抑制电极)。这些电极的显着变化将被强调,和相关的电化学技术的简要概述将给出。
    In high concentrations, ionic species can be toxic in the body, catalyzing unwanted bioreactions, inhibiting enzymes, generating free radicals, in addition to having been associated with diseases like Alzheimer\'s and cancer. Although ionic species are ubiquitous in the environment in trace amounts, high concentrations of these metals are often found within industrial and agricultural waste runoff. Therefore, it remains a global interest to develop technologies capable of quickly and accurately detecting trace levels of ionic species, particularly in aqueous environments that naturally contain other competing/inhibiting ions. Herein, we provide an overview of the technologies that have been developed, including the general theory, design, and benefits/challenges associated with ion-selective electrode technologies (carrier-doped membranes, carbon-based varieties, enzyme inhibition electrodes). Notable variations of these electrodes will be highlighted, and a brief overview of associated electrochemical techniques will be given.
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  • 文章类型: Journal Article
    The purpose of this review is to bring to the attention of the wider research community how two quite different optical sensory techniques were integrated resulting in a sensor device of exceptional sensitivity with wide ranging capability. Both authors have collaborated over a 20 year period, each researching initially surface plasmon resonance (SPR) and optical fibre Bragg grating devices. Our individual research, funded in part by EPSRC and industry into these two areas, converged, resulting in a device that combined the ultra-sensitive working platform of SPR behavior with that of fibre Bragg grating development, which provided a simple method for SPR excitation. During this period, they developed a new approach to the fabrication of nano-structured metal coatings for plasmonic devices and demonstrated on fibre optic platform, which has created an ultra-sensitive optical sensing platform. Both authors believe that the convergence of these two areas will create opportunities in detection and sensing yet to be realised. Furthermore, giving the reader \"sign-post\" research articles to help to construct models to design sensors and to understand their experimental results.
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  • 文章类型: Journal Article
    随着传统能源的消耗,满足能源需求的可持续能源在现代社会中非常重要。生物能源,水电,太阳能,风能是一些完善的可再生能源,有助于实现对兆瓦到千兆瓦电力规模的能源需求。基于纳米能源的纳米发电机是促进自供电系统的不断发展的技术,传感器,以及在物联网(物联网)蓬勃发展的时代中的灵活和便携式电子产品。纳米发电机可以从周围环境和周围环境中获取小规模的能量,以便有效利用。纳米发电机是基于压电的,Tribo,和热电效应,第一个此类产品是由Wang等人在2006年开发的。纳米发电机的发明是环境能量收集技术领域的突破,因为它们重量轻,容易制造,可持续,和无忧无虑的系统。在本文中,对基本面进行全面审查,性能,最近的事态发展,以及纳米发电机在自供电传感器中的应用,风能收集,蓝色能量收集,并讨论了其与太阳能光伏的集成。最后,还概述了该技术发展的前景和挑战。
    A sustainable power source to meet the needs of energy requirement is very much essential in modern society as the conventional sources are depleting. Bioenergy, hydropower, solar, and wind are some of the well-established renewable energy sources that help to attain the need for energy at mega to gigawatts power scale. Nanogenerators based on nano energy are the growing technology that facilitate self-powered systems, sensors, and flexible and portable electronics in the booming era of IoT (Internet of Things). The nanogenerators can harvest small-scale energy from the ambient nature and surroundings for efficient utilization. The nanogenerators were based on piezo, tribo, and pyroelectric effect, and the first of its kind was developed in the year 2006 by Wang et al. The invention of nanogenerators is a breakthrough in the field of ambient energy-harvesting techniques as they are lightweight, easily fabricated, sustainable, and care-free systems. In this paper, a comprehensive review on fundamentals, performance, recent developments, and application of nanogenerators in self-powered sensors, wind energy harvesting, blue energy harvesting, and its integration with solar photovoltaics are discussed. Finally, the outlook and challenges in the growth of this technology are also outlined.
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