肿瘤切除后由于肿瘤复发和感染引起的复杂伤口修复提出了重大的临床挑战。在这项研究中,一种双功能纳米复合免疫水凝胶敷料,SerMA-LJC,旨在解决与修复受感染的受损组织和预防肿瘤复发相关的问题。具体来说,免疫敷料由甲基丙烯酸酐修饰的丝胶(SerMA)和含有lonidamine(Lon)的自组装纳米颗粒(LJC)组成,JQ1,和氯e6(Ce6)。体外和体内实验表明,纳米复合水凝胶敷料可以触发免疫原性细胞死亡(ICD)并具有有效的抗肿瘤作用。此外,这种敷料可以缓解肿瘤细胞的酸性微环境,抑制肿瘤细胞表面PD-L1的过表达,从而改变免疫抑制肿瘤微环境并增强抗肿瘤免疫应答。Further,RNA测序分析表明,水凝胶敷料显着影响与免疫应答正调节相关的途径,凋亡过程,和其他相关途径,从而引发有效的抗肿瘤免疫反应。更重要的是,敷料产生大量的活性氧(ROS),能有效杀灭金黄色葡萄球菌,促进感染性伤口愈合。总之,这种双功能纳米复合免疫水凝胶敷料在预防肿瘤复发和促进感染性伤口愈合方面具有前景。
Complex wound repair due to tumor recurrence and infection following tumor resection presents significant clinical challenges. In this study, a
bifunctional nanocomposite immune hydrogel dressing, SerMA-LJC, is developed to address the issues associated with repairing infected damaged tissues and preventing tumor recurrence. Specifically, the immune dressing is composed of methacrylic anhydride-modified sericin (SerMA) and self-assembled nanoparticles (LJC) containing lonidamine (Lon), JQ1, and chlorine e6 (Ce6). In vitro and in vivo experiments demonstrate that the nanocomposite hydrogel dressing can trigger immunogenic cell death (ICD) and has a potent anti-tumor effect. Moreover, this dressing can mitigate the acidic microenvironment of tumor cells and suppress the overexpression of PD-L1 on the tumor cell surface, thereby altering the immunosuppressive tumor microenvironment and augmenting the anti-tumor immune response. Further, the RNA sequencing analysis revealed that the hydrogel dressing significantly impacts pathways associated with positive regulation of immune response, apoptotic process, and other relevant pathways, thus triggering a potent anti-tumor immune response. More importantly, the dressing generates a substantial amount of reactive oxygen species (ROS), which can effectively kill Staphylococcus aureus and promote infectious wound healing. In conclusion, this dual-function nanocomposite immune hydrogel dressing exhibits promise in preventing tumor recurrence and promoting infectious wound healing.