背景:这项研究的目的是确定光合装置的实际状态,并在受到不同光照强度(LI)影响的不同苗龄的绿华M.wakensis植物的叶绿素荧光(ChlF)成分中表现出明显的差异。选择盆栽的6个月大的温室幼苗和田间收集的具有5厘米高度的2.4岁幼苗,并随机分为7组,以50、100(分配为低LI)进行光合作用测量,300、500、1,000(作为中等LI),1,500和2,000(高LI)μmolm-2s-1光合光子通量密度(PPFD)处理。
结果:在6个月大的幼苗中,随着LI从50增加到2,000PPFD,非光化学猝灭和光抑制猝灭(qI)的值增加,但PSII的潜在量子效率(Fv/Fm)和光系统II的光化学效率(ΦPSII)值降低。在高LI条件下,在2.4岁的幼苗中观察到高电子传输速率和通过Fv/Fm值计算的实际PSII效率百分比。此外,在低LI条件下检测到较高的ΦPSII,具有较低的能量依赖性猝灭(qE)和QI值和光抑制率也降低。然而,在高LI处理下,qE和qI随着ΦPSII的降低而增加,光抑制%增加。
结论:这些结果可能有助于预测在受控环境和开放领域中生长的Mahonia物种的生长和分布变化,并具有不同光照的各种组合,和生态监测它们的恢复和栖息地的建立对于种源保护很重要,并有助于为幼苗制定更好的保护策略。
BACKGROUND: The aim of this study was to determine the actual state of the photosynthetic apparatus and exhibit distinguishable differences in the chlorophyll fluorescence (ChlF) components in different seedling ages of M. oiwakensis plants subjected to different light intensity (LI). Potted 6-month-old greenhouse seedlings and field collected 2.4-year-old seedlings with 5 cm heights were selected and randomly separated into seven groups for photosynthesis measurements illuminated with 50, 100 (assigned as low LI), 300, 500, 1,000 (as moderate LI), 1,500 and 2,000 (as high LI) μmol m-2 s-1 photosynthetic photon flux density (PPFD) treatments.
RESULTS: n 6-month-old seedlings, as LI increased from 50 to 2,000 PPFD, the values of non-photochemical quenching and photo-inhibitory quenching (qI) increased but potential quantum efficiency of PSII (Fv/Fm) and photochemical efficiency of photosystem II (ΦPSII) values decreased. High electron transport rate and percentage of actual PSII efficiency by Fv/Fm values were observed in 2.4-year-old seedlings at high LI conditions. Furthermore, higher ΦPSII was detected under low LI conditions, with lower energy-dependent quenching (qE) and qI values and photo-inhibition % decreased as well. However, qE and qI increased as ΦPSII decreased and photo-inhibition% increased under high LI treatments.
CONCLUSIONS: These results could be useful for predicting the changes in growth and distribution of Mahonia species grown in controlled environments and open fields with various combinations of varying light illuminations, and ecological monitoring of their restoration and habitat creation is important for provenance conservation and helps to formulate better conservation strategies for the seedlings.