糖胺聚糖(GAG)由于其重要的生物医学功能而发挥着至关重要的作用。硫酸软骨素(CS)和硫酸皮肤素(DS),GAG的主要代表家族,从石榴鱼(BeloneBelone)副产品中提取和纯化,即,蒙皮(GSB),骨骼(GCB),和头(GHB),并研究了它们的组成和抗凝血活性。CS/DS通过离子交换色谱法纯化,头收率为8.1%,3.7%的皮肤,骨骼为1.4%。还探索了醋酸纤维素电泳以分析提取的CS/DS。有趣的是,GHB,GSB,GCB的硫酸盐含量为21±2%,20±1%,和20±1.5%,分别。理化分析表明,硫酸盐的方差之间没有显着差异(p>0.05),糖醛酸,和从鱼的不同部位提取的GAG中的总糖。SAX-HPLC二糖分析表明,GSB和GCB主要由ΔDi-4S[ΔUA-GalNAc6S]组成(74.78%和69.22%,分别)和ΔDi-2,4S[ΔUA2S-GalNAc4S](10.92%和6.55%,分别)。然而,GHB由25.55%ΔDi-6S[ΔUA-GalNAc6S]和6.28%ΔDi-2,6S[ΔUA2S-GalNAc4S]组成。此外,经典抗凝试验也用于测量其体外抗凝特性,其中包括活化的部分凝血活酶时间,凝血酶原时间,和凝血酶时间。从石斑鱼副产品中分离的CS/DS表现出有效的抗凝血作用。根据这项研究,纯化的CS/DS显示出优异的抗凝血性能,可以被认为是具有抗凝血性能的新药。
Glycosaminoglycans (GAGs) play a crucial role due to their significant biomedical functions. Chondroitin sulfate (CS) and dermatan sulfate (DS), the main representative family of GAGs, were extracted and purified from garfish (Belone belone) by-products, i.e., skin (GSB), bones (GCB), and heads (GHB), and their composition and anticoagulant activity were investigated. CS/DS were purified by ion-exchange chromatography with yields of 8.1% for heads, 3.7% for skin, and 1.4% for bones. Cellulose acetate electrophoresis was also explored for analyzing the extracted CS/DS. Interestingly, GHB, GSB, and GCB possessed sulfate contents of 21 ± 2%, 20 ± 1%, and 20 ± 1.5%, respectively. Physico-chemical analysis showed that there were no significant differences (p > 0.05) between the variances for sulfate, uronic acid, and total sugars in the GAGs extracted from the different parts of fish. Disaccharide analysis by SAX-HPLC showed that the GSB and GCB were predominately composed of ΔDi-4S [ΔUA-GalNAc 6S] (74.78% and 69.22%, respectively) and ΔDi-2,4S [ΔUA2S-GalNAc 4S] (10.92% and 6.55%, respectively). However, the GHB consisted of 25.55% ΔDi-6S [ΔUA-GalNAc 6S] and 6.28% ΔDi-2,6S [ΔUA2S-GalNAc 4S]. Moreover, classical anticoagulation tests were also used to measure their anticoagulant properties in vitro, which included the activated partial thromboplastin time, prothrombin time, and thrombin time. The CS/DS isolated from garfish by-products exhibited potent anticoagulant effects. The purified CS/DS showed exceptional anticoagulant properties according to this research and can be considered as a new agent with anticoagulant properties.