背景:部分党参属植物(山西地名:鲁当)长期以来表现出很高的药用和经济价值。党参,党参的干燥根(Franch。)南夫。(C.pilosula),党参变种。莫德斯塔(南夫。)L.D.Shen(C.pilosulavar.modesta),或党参亚种。唐神(奥利夫.)D.Y.洪(C.pilosula亚种。汤神),早在清朝的本草丛心就被记录为中药。党参,一种获得中国国家地理标志认证的珍贵草药,以健脾而闻名,滋养肺,促进血液循环,并产生流体特性。鉴于慢性脑缺血(CCI)通常与气血不足和液体消耗的症状有关,我们探讨了党参汤治疗CCI的潜力。
目的:观察党参汤对双侧颈动脉闭塞大鼠术后脑血流量和认知功能的影响;探讨党参汤是否能减轻2-VO术后大鼠脑组织病理损伤;评估党参汤对趋化因子表达的影响。缺氧诱导因子,2-VO手术后大鼠的炎症介质。
方法:我们使用2-VO大鼠模型来模拟CCI。我们使用激光散斑成像(LSI)系统观察手术前后CBF的变化。目的是检查2-VO手术后不同时间点的CBF变化。四个星期,大鼠口服党参汤,剂量为2.7、5.4和10.8g/kg/天,或Ginaton,剂量为43.2mg/kg/天。评估党参对大鼠脑低灌注症状的影响。我们进行了莫里斯水迷宫(MWM),巴恩斯迷宫(BM),和前肢握力测试。此外,病理实验包括苏木精和伊红,Nissl,并进行Luxol快速蓝染色。此外,我们使用蛋白质印迹法检测趋化因子CKLF1和缺氧诱导因子1-α(HIF-1α)等蛋白质水平的变化.
结果:2-VO手术后一周,大鼠脑动脉供血迅速减少至术前水平的43.39%±3.53%。大脑皮层灌注在手术后24小时内达到最低点,术后第4周逐渐恢复稳定。BM结果的整合,MWM,和握力测试,评估2-VO手术后大鼠的认知功能和前肢力量,明确表明,党参治疗可显着减少MWM测试中的潜伏期并增加平台穿越次数。Ginaton表现出相当的效果。此外,Codonopsis和Ginaton都减少了错误的数量和在BM测试中定位目标孔所需的时间。组织病理学染色显示,党参和金纳多可以改善CCI后大鼠的病理损伤,并减少CKLF1和HIF-1α等因子的释放。
结论:党参汤对CCI大鼠具有保护作用,可能是通过调节趋化因子的水平,缺氧诱导因子,和神经炎症介质。
BACKGROUND: Some species of Codonopsis (local name in Shanxi: Ludang) have long demonstrated high medicinal and economic value. Radix Codonopsis, the dried root of Codonopsis pilosula (Franch.) Nannf. (C. pilosula), Codonopsis pilosula var. modesta (Nannf.) L.D.Shen (C. pilosula var. modesta), or Codonopsis pilosula subsp. tangshen (Oliv.) D.Y.Hong (C. pilosula subsp. tangshen), was recorded as a traditional Chinese medicine back in the Qing Dynasty in Ben Cao Cong Xin. Radix Codonopsis, a valuable medicinal herb certified by the Chinese National Geographic Indication, is known for invigorating the spleen, nourishing the lungs, promoting blood circulation, and generating fluid properties. Given that chronic cerebral ischemia (CCI) is often associated with the symptoms of qi and blood deficiencies and fluid depletion, we explored the potential of Codonopsis decoction in the treatment of CCI.
OBJECTIVE: We investigated the effects of Codonopsis decoction on cerebral blood flow (CBF) and cognitive function in rats with bilateral carotid artery occlusion after surgery; explored whether Codonopsis decoction alleviates pathological injuries in brain tissue of rats after 2-VO surgery; and assessed the impact of Codonopsis decoction on the expression of chemokines, hypoxia-inducible factors, and inflammatory mediators in rats after 2-VO surgery.
METHODS: We used a 2-VO rat model to simulate CCI. We used a laser speckle imaging (LSI) system to observe changes in CBF before and after surgery. The goal was to examine variations in CBF at different time points after 2-VO surgery. For 4 weeks, the rats were orally administered Codonopsis decoction at doses of 2.7, 5.4, and 10.8 g/kg/day, or Ginaton at a dose of 43.2 mg/kg/day. To assess the effect of Codonopsis on cerebral hypoperfusion symptoms in rats, we conducted the Morris water maze (MWM), Barnes maze (BM), and forelimb grip strength tests. Additionally, pathological experiments including hematoxylin and eosin, Nissl, and Luxol fast blue staining were conducted. Furthermore, we used western blotting to detect changes in the levels of proteins such as the chemotactic factor CKLF1 and hypoxia-inducible actor 1-alpha (HIF-1α).
RESULTS: One week after 2-VO surgery, cerebral arterial blood supply in the rats rapidly reduced to approximately 43.39% ± 3.53% of the preoperative level. Cerebral cortex perfusion reached its nadir within 24 h of surgery, gradually recovering and stabilizing by the fourth week after surgery. An integration of the results from the BM, MWM, and grip strength tests, which assessed cognitive function and forelimb strength in rats after 2-VO surgery, unequivocally revealed that Codonopsis treatment significantly reduced the latency period and increased the number of platform crossings in the MWM test. Ginaton exhibited a comparable effect. Moreover, both Codonopsis and Ginaton decreased the number of errors and the time required to locate the target hole in the BM test. Histopathological staining revealed that Codonopsis and Ginaton could ameliorate pathological damage in rats after CCI and reduce the release of factors such as CKLF1 and HIF-1α.
CONCLUSIONS: Codonopsis decoction exerted its protective effects on CCI rats possibly by modulating the levels of chemokines, hypoxia-inducible factors, and neuroinflammatory mediators.