背景:本仁灵芝是油棕的一种植物病原体,引起基底和上部茎腐病。
方法:基因组序列被用作参考,以研究在饥饿的碳(C)和氮(N)环境中生长过程中的基因表达,糖和木屑最少作为初始能源。进行这项研究是为了模拟油棕种植园中G.boninense生长过程中C-N营养来源的可能局限性。
结果:从西马来西亚的棕榈树中收集的分离物的基因组测序产生了67.12Mb的组装,编码19,851个预测基因。来自在这种饥饿培养基中生长期间的时间过程实验的转录组分析鉴定了差异表达的基因(DEGs),其被发现与29个代谢途径相关。在活跃的生长阶段,26个DEG与四个途径有关,包括次级代谢产物的生物合成,碳水化合物代谢,聚糖代谢和霉菌毒素生物合成。参与促进植物细胞壁降解的碳水化合物代谢途径的G.boninense基因被上调。有趣的是,与霉菌毒素生物合成途径相关的几个基因被鉴定为在病原体-宿主相互作用中起可能的作用。此外,代谢组学分析揭示了六种代谢物,麦芽糖,木二糖,低聚葡萄糖,甘氨酰脯氨酸,在时间过程实验的第2天上调的二甲基富马酸和阿拉伯糖醇。
结论:这项研究提供了有关G.boninense在代谢途径中表达的基因的信息,这些基因可能在宿主的初始感染中起作用。
BACKGROUND: Ganoderma boninense is a phytopathogen of oil palm, causing basal and upper stem rot diseases.
METHODS: The genome sequence was used as a reference to study gene expression during growth in a starved carbon (C) and nitrogen (N) environment with minimal sugar and sawdust as initial energy sources. This study was conducted to mimic possible limitations of the C-N nutrient sources during the growth of G. boninense in oil palm plantations.
RESULTS: Genome sequencing of an isolate collected from a palm tree in West Malaysia generated an assembly of 67.12 Mb encoding 19,851 predicted genes. Transcriptomic analysis from a time course experiment during growth in this starvation media identified differentially expressed genes (DEGs) that were found to be associated with 29 metabolic pathways. During the active growth phase, 26 DEGs were related to four pathways, including secondary metabolite biosynthesis, carbohydrate metabolism, glycan metabolism and mycotoxin biosynthesis. G. boninense genes involved in the carbohydrate metabolism pathway that contribute to the degradation of plant cell walls were up-regulated. Interestingly, several genes associated with the mycotoxin biosynthesis pathway were identified as playing a possible role in pathogen-host interaction. In addition, metabolomics analysis revealed six metabolites, maltose, xylobiose, glucooligosaccharide, glycylproline, dimethylfumaric acid and arabitol that were up-regulated on Day2 of the time course experiment.
CONCLUSIONS: This study provides information on genes expressed by G. boninense in metabolic pathways that may play a role in the initial infection of the host.