漆酶是在其结构中具有铜的放热酶,并且通过提供酚类化合物和芳香胺的氧化和分解木质素而在生物降解中具有重要作用。这项研究的目的是通过对从纺织厂处理污泥中分离出的Proteusmirabilis进行优化研究,以最小的成本和能量达到最大的漆酶活性。为了提高分离株的漆酶活性,通过研究碳(葡萄糖,果糖,醋酸钠,羧甲基纤维素,木糖)和氮源(硝酸钾,酵母提取物,来自大豆的蛋白胨,细菌蛋白胨),孵化时间,pH值,温度和硫酸铜(II)浓度,然后根据获得的结果。响应面法(RSM)对六个不同的变量进行了三个水平。根据从RSM获得的数据,在pH7.77,温度30.03oC时达到最大漆酶活性,0.5g/LCuSO4、0.5g/L果糖和0.082g/L酵母浸膏条件。毕竟,漆酶活性增加2.7倍。因此,通过优化研究可以提高奇异假单胞菌的漆酶活性。作为文献研究的结果获得的信息是,在实验室和工业规模中生产的漆酶是昂贵的并且它们的量是低的。这项研究对于以更少的成本和能量从奇异假单胞菌获得更多的漆酶活性是重要的。
Laccase is an exothermic enzyme with copper in its structure and has an important role in biodegradation by providing oxidation of phenolic compounds and aromatic amines and decomposing lignin. The aim of this study is to reach maximum laccase enzyme activity with minimum cost and energy through optimization studies of Proteusmirabilis isolated from treatment sludge of a textile factory. In order to increase the laccase enzyme activities of the isolates, medium and culture conditions were optimized with the study of carbon (Glucose, Fructose, Sodium Acetate, Carboxymethylcellulose, Xylose) and nitrogen sources (Potassium nitrate, Yeast Extract, Peptone From Soybean, Bacteriological Peptone), incubation time, pH, temperature and Copper(II) sulfate concentration then according to the results obtained. Response Surface Method (RSM) was performed on six different variables with three level. According to the data obtained from the RSM, the maximum laccase enzyme activity is reached at pH 7.77, temperature 30.03oC, 0.5 g/L CuSO4, 0.5 g/L fructose and 0.082 g/L yeast extract conditions. After all, the laccase activity increased 2.7 times. As a result, laccase activity of P. mirabilis can be increased by optimization studies. The information obtained as a result of the literature studies is that the laccase enzymes produced in laboratory and industrial scale are costly and their amounts are low. This study is important in terms of obtaining more laccase activity from P.mirabilis with less cost and energy.