鱼蛋白水解物(FPH)作为水产养殖中的膳食蛋白补充剂和免疫刺激剂显示出巨大的潜力,特别是在尼罗罗非鱼生产中。通过以不同的百分比(0%,0.5%,1%,和2%)。尼罗罗非鱼饲喂FPH饮食90天,以及他们的增长表现,饲料利用,血液生物化学,肝脏和肠道形态学,并对其耐药性进行了研究。研究结果表明,实验饮食组之间的饮食物理属性,例如颗粒耐久性指数和水稳定性,差异显着(p<0.05)。此外,当FPH包含在1%和2%时,试验饮食更可口.用2%FPH处理的饮食喂养的鱼具有比其他处理显著(p<0.05)更大的生长指数。此外,饲料利用率显著提高(p<0.05)。实验饮食和肠道细菌总数(TBC)表现出与FPH水平上升的趋势,其中2%FPH处理的饮食记录最高的TBC。中性粒细胞(109/L),淋巴细胞(109/L),嗜酸性粒细胞(109/L),2%FPH治疗组的红细胞(1012/L)计数显着(p<0.05)更高,而白细胞(109/L),和嗜碱性粒细胞(109/L)计数不受FPH纳入的影响。此外,FPH治疗组显示肌酐较低,胆红素,和尿素水平比对照。组织学检查表明,2%FPH喂养的尼罗罗非鱼的肠道上皮壁未破裂,更多绒毛频繁分布的杯状细胞,更宽的肌层,与其他处理相比,地层密实结合更强。此外,与相应组相比,该组表现出更多的细胞核和红细胞,而肝脏中的液泡细胞质更少。在细菌攻击测试期间,给予含有2%FPH的饮食的尼罗罗非鱼对S.iniae具有显著(p<0.05)更高的抗性(83.33%)。在饮食中FPH含量较高的情况下,观察到农场经济效率的显着提高(p<0.05)。总之,在尼罗罗非鱼日粮中添加2%FPH可以改善其生长性能,饲料利用,健康状况,抗病性,和农业经济效益。
Fish protein hydrolysate (FPH) has shown immense potential as a dietary protein supplement and immunostimulant in aquaculture, especially in Nile tilapia production. Four isoproteic diets (30% crude protein) were prepared by including FPH at varying percentages (0%, 0.5%, 1%, and 2%). Nile tilapia fed with FPH diets for 90 days, and their growth performance, feed utilization, blood biochemistry, liver and gut morphology, and resistance against Streptococcus iniae were investigated. The findings revealed that diets physical attributes such as pellet durability index and water stability were remarkably (p < 0.05) varied between experimental diet groups. Furthermore, the test diets were more palatable when FPH was included at 1% and 2%. Fish that were fed with a 2% FPH-treated diet had significantly (p < 0.05) greater growth indices than other treatments. Additionally, their feed utilization was significantly (p < 0.05) improved. The experimental diets and intestinal total bacteria count (TBC) exhibited a rising trend with FPH levels, where the 2% FPH-treated diet recorded the highest TBC. Neutrophil (109/L), lymphocyte (109/L), eosinophil (109/L), and red blood cell(1012/L) counts were significantly (p < 0.05) higher in the 2% FPH-treated group, while the white blood cell (109/L), and basophil (109/L) counts were not influenced by the FPH inclusion. Moreover, the FPH-treated groups displayed lower creatinine, bilirubin, and urea levels than the control. The histological examination demonstrated that themid-intestine of 2% FPH-fed Nile tilapia had an unbroken epithelial wall, more villi with frequent distribution of goblet cells, wider tunica muscularis, and stronger stratum compactum bonding than other treatments. Additionally, this group exhibited more nuclei and erythrocytes and less vacuolar cytoplasm in liver than their counterparts. Nile tilapia that were given a diet containing 2% FPH had significantly (p < 0.05) higher resistance (83.33%) to S. iniae during the bacterial challenge test. A significant (p < 0.05) enhancement in farm economic efficiency was observed in the higher inclusion of FPH in diets. In summary, 2% FPH supplementation in Nile tilapia diets improved their growth performance, feed utilization, health status, disease resistance, and farm economic efficiency.