背景:BolA基因家族包含三个成员,据报道,BolA家族成员2(BOLA2)的高表达与几种癌症的预后相关.然而,BOLA3与肺腺癌(LUAD)之间的关系尚不清楚.
方法:通过在线数据库分析BOLA3的表达。使用LinkedOmics进行共表达基因和基因集富集分析(GSEA)。使用受试者工作特征(ROC)曲线评估诊断价值。使用Prognoscan和Kaplan-Meier绘图仪分析BOLA3的预后价值。通过肿瘤免疫评估资源(TIMER)和单样本基因集富集分析(ssGSEA)来探讨BOLA3与肿瘤免疫浸润之间的关系。
结果:结果表明,BOLA3在LUAD中的表达明显高于正常组织。BOLA3的高表达与T期有关,N级,病理分期(均P<0.001)。此外,BOLA3表达升高与LUAD患者的总生存期(OS)和无进展生存期(PFS)相关(OS:HR=2.58,log-rankP=1.3e-11;PFS:HR=2.36,log-rankP=4.1e-05).BOLA3表达水平与B细胞浸润水平呈负相关,CD4+T细胞,巨噬细胞,中性粒细胞,和树突状细胞(DC)。GSEA分析显示BOLA3主要参与线粒体呼吸链复合物组装,翻译起始,等。结论:这些结果表明,在LUAD中,BOLA3上调与不良预后和免疫浸润有关,BOLA3可以作为潜在的免疫疗法靶标。
BACKGROUND: BolA gene family contains three members, high expression of BolA family member 2 (BOLA2) has been reported to be associated with prognosis of several cancers. However, the relationship between
BOLA3 and lung adenocarcinoma (LUAD) remains unclear.
METHODS: Expression of
BOLA3 was analyzed by online database. Co-expressed genes and gene set enrichment analysis (GSEA) were performed using LinkedOmics. Diagnostic value was assessed using receiver operating characteristic (ROC) curves. The prognostic value of
BOLA3 was analyzed using Prognoscan and Kaplan-Meier Plotter. The Tumor Immune Estimation Resource (TIMER) and Single-sample Gene Set Enrichment Analysis (ssGSEA) were used to explore the relationship between BOLA3 and tumor immune infiltration.
RESULTS: The results showed that the expression of BOLA3 was significantly higher in LUAD than in normal tissues. High expression of
BOLA3 was associated with T stage, N stage, pathologic stage (all P < 0.001). In addition, elevated expression of BOLA3 was associated with overall survival (OS) and progression-free survival (PFS) in LUAD ((OS:HR = 2.58, log-rank P = 1.3e - 11; PFS:HR = 2.36, log-rank P = 4.1e - 05). BOLA3 expression level has negative correlations with infiltrating levels of B cells, CD4 +T cells, macrophages, neutrophils, and dendritic cells (DCs). GSEA analysis showed BOLA3 joined mainly in mitochondrial respiratory chain complex assembly, translational initiation, etc. CONCLUSIONS: These results showed up-regulated in BOLA3 was correlated with poor prognosis and immune infiltrates in LUAD,
BOLA3 can be served as a potential immunotherapy target.