■在组织修复或再生过程中,几种生物活性分子被释放并相互作用,并作为复杂的添加剂或抑制剂用于组织重建。在这项研究中,与肿瘤坏死因子-α(TNF-α)抑制联合治疗的骨愈合效果,血管内皮生长因子A(VEGF-A)和骨形态发生蛋白-7(BMP-7)通过基因沉默释放,用磷酸钙纳米颗粒(CaP)在大鼠股骨头中进行组织学基因转染,形态学上,并进行了生化评估。
■携带编码BMP-7和VEGF的质粒DNA的CaP三重官能化糊状物),并开发了针对TNF-α的siRNA并表示为CaP3mix。为了比较3mixCaP的效果,CaP与质粒DNA编码BMP-7,VEGF,制备编码TNF-α的siRNA或siRNA,并分别表示为CaP/PEI/pBMP-7/SiO2、CaP/PEI/pVEGF/SiO2或CaP/PEI/siRNA-TNF-α/SiO2。在植入10天和21天后,研究了大鼠股骨头骨缺损中的骨愈合。
■骨形成相关标志物OCN的水平,10天后,在3mixCaP中,Runx2和SP7在蛋白质和基因水平上增加,与植入21天后的其他治疗相比,3mixCaP显着加速了骨愈合。
■装载编码BMP-7和VEGF的质粒DNA和编码TNF-α的siRNA的三重官能化CaP糊是用于骨组织修复的有前途的生物活性材料。
UNASSIGNED: During tissue repair or regeneration, several bioactive molecules are released and interact with each other and act as complex additives or inhibitors for tissue reconstruction. In this study, the bone-healing effects of the combination treatment with tumor necrosis factor-α (TNF-α) inhibition, vascular endothelial growth factor A (VEGF-A) and bone morphogenetic protein-7 (BMP-7) release by gene silencing, and gene transfection with calcium phosphate nanoparticles (CaP) in the rat femoral head was histologically, morphologically, and biochemically evaluated.
UNASSIGNED: A triple-functionalized paste of CaP carrying plasmid DNA encoding for BMP-7 and for VEGF), and siRNA against TNF-α was developed and denoted as CaP3mix. To compare the effects of 3mixCaP, CaP with plasmid DNA encoding BMP-7, VEGF, or siRNA encoding TNF-α was prepared and denoted as CaP/PEI/pBMP-7/SiO2, CaP/PEI/pVEGF/SiO2, or CaP/PEI/siRNA-TNF-α/SiO2, respectively. The bone healing in bone defects in the rat femoral head was investigated after 10 and 21 days of implantation.
UNASSIGNED: The levels of bone formation-related markers OCN, Runx2, and SP7 increased at the protein and gene levels in 3mixCaP after 10 days, and 3mixCaP significantly accelerated bone healing compared with the other treatments after 21 days of implantation.
UNASSIGNED: The triple-functionalized CaP paste loading plasmid DNA encoding BMP-7 and VEGF and siRNA encoding TNF-α is a promising bioactive material for bone tissue repair.