背景:囊状挛缩是涉及硅胶植入物的乳房手术后最常见的并发症之一。这种情况最可能的原因是生物膜形成。在这项研究中,研究了局部抗微生物疗法对植入物表面生物膜形成的功效。
方法:36只大鼠分为6组。在每只老鼠的背部形成三个口袋,和1×2厘米的植入物表面样品从光滑,聚氨酯和纹理植入物随机放入口袋。所有样品均接种表皮葡萄球菌。在第1-2-3组中,将接种的样品放入袋中,并在1、6和24小时后取出,分别。在第4-5-6组中,在1、6和24小时后放置并取出浸入利福霉素的接种样品,分别。用平板计数法测量细菌负荷。
结果:第4-5-6组的细菌负荷低于第1-2-3组(p<0.05)。在第4-5-6组中,在所有时间点(1、6和24小时;p<0.05),聚氨酯的细菌负荷低于纹理化表面。再一次,在第4-5-6组中,24小时光滑表面的细菌负荷低于纹理表面(p<0.05)。在第4-5-6组中,在所有时间点,聚氨酯的细菌负荷均低于光滑表面。但差异无统计学意义(1、6和24h;p<0.05)。
结论:结果表明,局部抗生素治疗可有效减少所有表面的细菌负荷。局部利福霉素在聚氨酯表面的有效性更高,活动的持续时间比其他表面长。
方法:本期刊要求作者为每篇文章分配一定程度的证据。为了完整描述这些循证医学评级,请参阅目录或在线作者说明www。springer.com/00266.
BACKGROUND: Capsular contracture is one of the most common complications after breast surgery involving silicone implants. The most likely cause of this condition is biofilm formation. In this study, the efficacy of local antibiotherapy against biofilm formation on implant surfaces was investigated.
METHODS: Thirty-six rats were divided into six groups. Three pockets were created on the dorsum of each rat, and 1 × 2 cm implant surface samples from smooth, polyurethane and textured implants were randomly placed into pockets. All samples were inoculated with staphylococcus epidermidis. In groups 1-2-3, inoculated samples were placed into the pockets and removed after 1, 6 and 24 h, respectively. In groups 4-5-6, inoculated samples immersed with rifamycin were placed and removed after 1, 6 and 24 h, respectively. Bacterial load was measured with plate count method.
RESULTS: Bacterial load was lower in groups 4-5-6 than in groups 1-2-3 (p < 0.05). In groups 4-5-6, bacterial load was lower for polyurethane than for textured surfaces at all time points (1, 6 and 24 h; p < 0.05). Again, in groups 4-5-6, bacterial load was lower for smooth than for textured surfaces at 24 h (p < 0.05). In groups 4-5-6, bacterial load was lower for polyurethane than for smooth surfaces at all time points, but difference was not statistically significant (1, 6 and 24 h; p < 0.05).
CONCLUSIONS: The results suggest that local antibiotic therapy was effective in reducing the bacterial load on all surfaces. The effectiveness of local rifamycin on the polyurethane surface was higher, and the duration of activity was longer than other surfaces.
METHODS: This journal requires that authors assign a level of evidence to each article. For a full description of these evidence-based medicine ratings, please refer to the Table of contents or the online Instructions to Authors www.springer.com/00266 .