对乙酰氨基酚(APAP)由于其解热和镇痛活性而用作主要药物。APAP在肝脏中的毒性作用机制是由于谷胱甘肽的消耗引起自由基的产生。因此,我们的工作目的是研究APAP引起的肝损伤及其通过肉桂油(CO)的自由基清除活性对雄性Wistar大鼠的修复作用。为了研究不同剂量(50、100和200mg/kgb.w.)的CO的作用,在12:00-1:00PM之间每天给予动物单次口服剂量的CO,持续14天。生化的变化,氧化标志物失衡,白细胞介素,确定了caspases和组织病理学研究以定量CO的肝保护作用。一剂APAP(2g/kgb.w.)导致显著的肝毒性,并显著增加血清标志物丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST),碱性磷酸酶(ALP),胆红素,白蛋白,总蛋白质,脂质过氧化(LPO)的含量,白细胞介素(IL-1β,IL-6),caspase-3,-9表达,观察到DNA片段化和组织病理学变化。LPO水平显著下降,白细胞介素类IL-1β,IL-6,caspase-3,-9表达,通过剂量依赖性地施用CO,可以逆转DNA片段的定性和定量测定以及组织病理学变化。此外,它还恢复了抗氧化酶的活性。我们的研究表明,通过用CO治疗动物,可以恢复APAP引起的肝脏氧化参数失衡。
Acetaminophen (APAP) is used as a primary drug due to its antipyretic and analgesic activity. The mechanism of action of APAP toxicity in the liver is due to the depletion of glutathione which elicited free radicals generation. Therefore, the objective of our work is to investigate the APAP induced liver damage and its repair by free radical scavenging activity of cinnamon oil (CO) in male Wistar rats. To investigate the effects of CO at different doses (50, 100 and 200 mg/kg b.w.), animals were given a single oral dose of CO per day for 14 days between 12:00-1:00 PM. The biochemical changes, imbalance in oxidative markers, interleukins, caspases and histopathological studies were determined for quantifying the hepatoprotective effect of CO. One dose of APAP (2 g/kg b.w.) results in significant hepatotoxicity and marked increase the serum markers alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, albumin, total protein, content of lipid peroxidation (LPO), interleukins (IL-1β, IL-6), caspase-3, -9 expression, DNA fragmentation and histopathological changes were observed. Significant decrease in the levels of LPO, interleukins IL-1β, IL-6, caspase-3, -9 expressions, qualitative as well as quantitative determination of DNA fragments and histopathological changes were reversed by the administration of CO dose dependently. Furthermore, it also restores the depleted activity of antioxidative enzymes. Our study shows that an imbalance in the oxidative parameter in the liver by APAP is restored by treating the animals with CO.