背景:生物素羧基载体蛋白(BCCP)是乙酰辅酶A-羧化酶(ACCase)的亚基,其在脂肪酸从头生物合成过程中的关键步骤中催化乙酰辅酶A向丙二酰辅酶A的转化。脂质,脂质代谢物,已知脂质代谢和修饰酶在植物的生物和非生物胁迫耐受性中起作用。在这方面,了解甘蓝型油菜BCCP基因将有助于提高油菜的生物和非生物胁迫耐受性。
结果:在这项研究中,根据已发表的基因组数据,我们在5种芸苔属植物中鉴定出43个BCCP基因。其中,芸苔属油菜,甘蓝,黑油菜,甘蓝型油菜和芥菜有六个,七、七、10和13个BCCP同源物,分别。系统发育分析将它们分为五类,每个都有独特的保守域。通过顺式元件分析确定,所有BCCP基因的启动子区域均包含与胁迫相关的顺式作用元件。我们分别在甘蓝型油菜和甘蓝型油菜中鉴定了四个和三个重复的基因对(节段),表明分段复制在该基因家族扩展中的作用。拟南芥和五个芸苔属植物的直系同源基因对的Ka/Ks比值大多小于1.0,这意味着纯化选择,即,选择性去除有害等位基因,在芸苔属基因组的进化过程中发挥了作用。使用qRT-PCR分析10个BnaBCCP基因显示出不同的表达模式,因为植物暴露于生物胁迫,如根肿病和菌核病,和干旱等非生物胁迫,低温和盐度胁迫。
结论:芸苔属植物BCCPs的鉴定和功能分析表明,其中一些基因可能在生物和非生物胁迫反应中起重要作用。本研究结果为更好地理解这些基因为提高芸苔属作物的抗逆性奠定了基础。
BACKGROUND: Biotin carboxyl carrier protein (
BCCP) is a subunit of Acetyl CoA-carboxylase (ACCase) which catalyzes the conversion of acetyl-CoA to malonyl-CoA in a committed step during the de novo biosynthesis of fatty acids. Lipids, lipid metabolites, lipid-metabolizing and -modifying enzymes are known to play a role in biotic and abiotic stress tolerance in plants. In this regard, an understanding of the Brassica napus
BCCP genes will aid in the improvement of biotic and abiotic stress tolerance in canola.
RESULTS: In this study, we identified 43
BCCP genes in five Brassica species based on published genome data. Among them, Brassica rapa, Brassica oleracea, Brassica nigra, Brassica napus and Brassica juncea had six, seven, seven, 10 and 13
BCCP homologs, respectively. Phylogenetic analysis categorized them into five classes, each with unique conserved domains. The promoter regions of all
BCCP genes contained stress-related cis-acting elements as determined by cis-element analysis. We identified four and three duplicated gene pairs (segmental) in B. napus and B. juncea respectively, indicating the role of segmental duplication in the expansion of this gene family. The Ka/Ks ratios of orthologous gene pairs between Arabidopsis thaliana and five Brassica species were mostly less than 1.0, implying that purifying selection, i.e., selective removal of deleterious alleles, played a role during the evolution of Brassica genomes. Analysis of 10 BnaBCCP genes using qRT-PCR showed a different pattern of expression because of exposure of the plants to biotic stresses, such as clubroot and sclerotinia diseases, and abiotic stresses such as drought, low temperature and salinity stresses.
CONCLUSIONS: The identification and functional analysis of the Brassica BCCPs demonstrated that some of these genes might play important roles in biotic and abiotic stress responses. Results from this study could lay the foundation for a better understanding of these genes for the improvement of Brassica crops for stress tolerance.