已知含有B3结构域的转录因子(TF)在各种发育过程中发挥重要作用。包括胚胎发生,种子萌发,等。杨树B3TF超家族的特征和功能研究仍然有限,尤其是它们在木材形成中的作用。在这项研究中,我们对白杨×甘杜叶杨中B3TF基因进行了全面的生物信息学分析和表达分析。在该杂交杨树的基因组中总共鉴定出160个B3TF基因,和它们的染色体位置,同伦关系,基因结构,和启动子顺式作用元件进行了分析。通过结构域结构和系统发育关系分析,这些蛋白质被分为四个家族LAV,RAV,ARF,和REM。结构域和保守性分析揭示了家族之间不同的基因数量和不同的DNA结合结构域。合成关系分析表明,大约87%的基因来自基因组重复(分段或串联)。促进了白杨×甘杜洛萨B3家族的扩张。七个物种的系统发育揭示了B3TF基因在不同物种之间的进化关系。在分化木质部中高表达的18种蛋白质中的B3结构域具有较高的同系性,暗示了这七个物种的共同祖先.我们对两个不同年龄的杨树的代表性基因进行了共表达分析,其次是路径分析。在与四个B3基因共表达的基因中,14参与木质素合酶和次生细胞壁生物合成,包括PagCOMT2、PagCAD1、PagCCR2、PagCAD1、PagCCoAOMT1、PagSND2和PagNST1。我们的结果为杨树中的B3TF家族提供了有价值的信息,并显示了B3TF基因在工程中改善木材特性的潜力。
B3-domain containing transcription factors (TFs) are well known to play important roles in various developmental processes, including embryogenesis, seed germination, etc. Characterizations and functional studies of the
B3 TF superfamily in poplar are still limited, especially on their roles in wood formation. In this study, we conducted comprehensive bioinformatics and expression analysis of
B3 TF genes in Populus alba × Populus glandulosa. A total of 160
B3 TF genes were identified in the genome of this hybrid poplar, and their chromosomal locations, syntenic relationships, gene structures, and promoter cis-acting elements were analyzed. Through domain structure and phylogenetic relationship analyses, these proteins were classified into four families LAV, RAV, ARF, and REM. Domain and conservation analyses revealed different gene numbers and different DNA-binding domains among families. Syntenic relationship analysis suggested that approximately 87% of the genes resulted from genome duplication (segmental or tandem), contributing to the expansion of the B3 family in P. alba × P. glandulosa. Phylogeny in seven species revealed the evolutionary relationship of B3 TF genes across different species. B3 domains among the eighteen proteins that were highly expressed in differentiating xylem had a high synteny, suggesting a common ancestor for these seven species. We performed co-expression analysis on the representative genes in two different ages of poplar, followed by pathways analysis. Among those genes co-expressed with four B3 genes, 14 were involved in lignin synthases and secondary cell walls biosynthesis, including PagCOMT2, PagCAD1, PagCCR2, PagCAD1, PagCCoAOMT1, PagSND2, and PagNST1. Our results provide valuable information for the
B3 TF family in poplar and show the potential of
B3 TF genes in engineering to improve wood properties.