布鲁氏菌病是一种世界范围内分布的人畜共患传染病。这项横断面研究旨在确定反刍动物的牛奶和血液样本中布鲁氏菌的发生以及与伊朗东部动物布鲁氏菌病相关的重要因素。共200个配对样本,包括血液(100)和牛奶(100),是从山羊那里获得的,绵羊,和伊朗东部的奶牛。对血清进行血清凝集(SAT)和2-巯基乙醇(2-ME)测试。进行多重聚合酶链反应(m-PCR)测定以鉴定以下布鲁氏菌,包括B.abortusbiovar1、2、4、3b,5、6和9,B.abortusS19,B.melitensis,和B.melitensisRev.1疫苗株。还在第二次PCR测定中研究了流产芽孢杆菌RB51疫苗株。布鲁氏菌感染的危险因素。包括堕胎的影响,接触野生动物,牧群类型,年龄,和先前的疫苗接种预测血液污染的B.abortusbiovar1、2和4是通过使用人工神经网络建模的。关于SAT和2-ME测试,总共23个样本为血清阳性。总的来说,在35%和15%的血液和牛奶样本中检测到流产芽孢杆菌,分别,通过m-PCR分析。检测到牛奶和血液中的每一种的一个样品中具有B.melitensis。一些样品同时被两种布鲁氏菌属或两种流产芽孢杆菌生物制品污染。在牛奶和血液样品中还检测到流产芽孢杆菌S19和B.melitensisRev.1疫苗株。ANN模型的灵敏度和特异度分别为81%和62%,分别。总之,B.abortus在血液和牛奶样品中的频率高于B.melitensis。ANN确定的羊群类型,以前的疫苗接种,和动物的年龄是流产芽孢杆菌血液污染的最大预测因子。
Brucellosis is a zoonotic infectious disease with a worldwide distribution. This cross-sectional study aimed to determine the occurrence of Brucella in milk and blood samples of ruminants and the importance factors associated with animal brucellosis in Eastern Iran. A total of 200 paired samples, including blood (100) and milk (100), were obtained from the goats, sheep, and cows in Eastern Iran. Serum agglutination (SAT) and 2-mercapto ethanol (2-ME) tests were performed on the sera. A multiplex-polymerase chain reaction (m-PCR) assay was performed to identify the following species of Brucella, including B. abortus biovar 1, 2, 4, 3b, 5, 6, and 9, B. abortus S19, B. melitensis, and B. melitensis Rev.1 vaccine strain. B. abortus RB51 vaccine strain was also investigated in a second PCR assay. Risk factors for infection with Brucella spp. including the effect of abortion, contact with the wild animals, herd type, age, and previous vaccination in predicting blood contamination with B. abortus biovar 1, 2, and 4 were modeled by use of the artificial neural network. A total of 23 samples were seropositive regarding SAT and 2-ME tests. In total, B. abortus was detected in 35% and 15% of blood and milk samples, respectively, by the m-PCR assay. One sample of each of milk and blood was detected to have B. melitensis. Some samples were simultaneously contaminated with two Brucella species or two biovars of B. abortus. B. abortus S19 and B. melitensis Rev.1 vaccine strains were also detected in milk and blood samples. The sensitivity and specificity of the ANN model were 81% and 62%, respectively. In conclusion, B. abortus had higher frequency than B. melitensis in blood and milk samples. ANN determined herd type, previous vaccination, and age of the animal as the largest predictors of blood contamination with B. abortus.