形态发生,伤口愈合,一些癌症转移取决于细胞群的迁移,这些细胞群需要被引导到目的地以及与其他细胞运动协调。在斑马鱼原肠胚形成过程中,胚胎轴的延伸是由向动物极迁移的中内胚层polster引导的,其次是经历收敛和延伸的轴向中胚层。这里,我们研究了polster细胞如何被引导到动物极点。使用精确激光烧蚀的组合,先进的移植,和功能以及硅方法,我们确定每个polster细胞都是由其直接跟随细胞定向的。每个细胞都感知追随者的迁移,通过E-钙粘蛋白/α-连环蛋白机械转导,并与他们保持一致。因此,定向信息在整个组织中从细胞传播到细胞。跟随者对迁移细胞的这种指导确保了运动的长期协调和发育稳健性。
Morphogenesis, wound healing, and some cancer metastases depend upon the migration of cell collectives that need to be guided to their destination as well as coordinated with other cell movements. During zebrafish gastrulation, the extension of the embryonic axis is led by the mesendodermal polster that migrates toward the animal pole, followed by the axial mesoderm that undergoes convergence and extension. Here, we investigate how polster cells are guided toward the animal pole. Using a combination of precise laser ablations, advanced transplants, and functional as well as in silico approaches, we establish that each polster cell is oriented by its immediate follower cells. Each cell perceives the migration of followers, through E-cadherin/α-catenin mechanotransduction, and aligns with them. Therefore, directional information propagates from cell to cell over the whole tissue. Such guidance of migrating cells by followers ensures long-range coordination of movements and developmental robustness.